〔Abstract〕 Objective To observe the expression of miR-214-3 p in gastric cancer tissues, explore its effect on the metastatic ability of gastric cancer cells, and explore the mechanism of its role. Methods The expression of miR-214-3 p in 41 pairs of gastric cancer and adjacent normal tissues and its relationship with tumor clinicopathological characteristics were detected by Real-time quantitative PCR; gastric cancer cell MGC-803 was inoculated in culture dishs, with 3 replicate wells in each group. The experiment was divided into 3 groups: miR-214-3 p up-regulated group(transfected miR-214-3 p mimic), miR-214-3 p down-regulated group(transfected miR-214-3 p inhibitor), and a NC group was set respectively; Real-time quantitative PCR was used to detect the expression levels of p53 in each group, and the Transwell experiment was used to detect the migration and invasion ability of MGC-803 cells after transfection with miR-214-3 p inhibitor. Bioinformatics analysis and luciferase activity determination determine whether p53 is the target gene of miR-214-3 p. Results Compared with adjacent tissues, miR-214-3 p was up-regulated in gastric cancer tissues and was associated with tumor lymph node metastasis; compared with the NC group, the expression of p53 in the miR-214-3 p up-regulated group decreased, and the expression of p53 increased in the miR-214-3 p down-regulated group; the results of Transwell experiment showed that compared with the NC group, cells of the miR-214-3 p down-regulated group had lower migration and invasion capabilities; bioinformatics analysis and luciferase activity measurement results showed that miR-214-3 p inhibited p53 expression by targeting its 3′UTR. Conclusion miR-214-3 p may play an important role in gastric cancer development and progression, and miR-214-3 p can be used as a potential targeted biomark for gastric cancer.