〔Abstract〕 Objective To observe the differential expression of ferroportin(FPN) in non-small cell lung cancer(NSCLC) and to explore the mechanism of FPN effect on NSCLC cell migration and proliferation. Methods Immunohistochemistry(IHC) was used to detect the expression level of FPN in 60 cases of lung cancer and 20 cases of normal lung tissue. Western blot was used to detect the expression level of FPN in cell lines.FPN overexpression system(PCDH-FPN-F) and knockdown system(shFPN) were constructed respectively, and verified by Western blot and qRT-PCR.The effect of FPN on lung cancer cell migration was detected by scratch test.Growth curve method was used to detect the effect of FPN on proliferation of lung cancer cells. Results Compared with normal lung tissue, the expression of FPN in lung cancer tissues significantly decreased(P=0.000). Compared with normal lung cell lines, the expression of FPN in lung cancer cell lines significantly decreased(P<0.000 1).Compared with the control group, the expression of FPN in the overexpressed group significantly increased(Western blot: P<0. 01,qRT-PCR: P<0. 000 1). Compared with the control group,FPN expression significantly decreased in the knockdown group( Western blot: P<0. 001,qRT-PCR: P<0. 001). After overexpression of FPN,the wound healing distance between cell scratches was significantly lower than that of the control group at 18 h and 36 h. The number of cells in 2 d and 4 d was significantly lower than that in the control group. When FPN was knocked down,the wound healing distance between cell scratches at 18 h and 36 h was significantly higher than that of the control group. The number of cells in 2 d and 4 d was significantly higher than that in the control group. Conclusion After low expression of FPN,the migration and proliferation of lung cancer cells were enhanced,which may be the reason for the differential expression of FPN in lung cancer.