〔Abstract〕 Objective To investigate the effects of miR-30 a-5 p on the proliferation, migration, cycle and apoptosis of laryngeal squamous cell carcinoma cells by targeting the regulation of MTA1. Methods The target gene MTA1 of miR-30 a-5 p was predictedby biological information technology and verified by RT-qPCR and dual-luciferase reporting experiment. miR-30 a-5 p mimics and NC were transfected into LSCC cell line Hep2 at logarithmic phase. Cell proliferation was detected by CCK-8 assay. Transwell assay was used to detect cell migration. Flow cytometry was used to detect cell cycle and apoptosis. Results In the real-time quantitative PCR results, the post-transfection expression levels of experimental group and control group were(12.62±0.97) and(1.04±0.32), respectively, with statistically significant difference(P<0.05). In the results of the double luciferase report experiment, the experiment levels of miR-30 a-5 p mimics+ pGL4.10+pGL 4.73 were(0.41±0.06) and(0.91±0.01), respectively, when compared with the wild-type and mutant-type, and the differencewas statistically significant(P<0.05).In the results of CCK-8 experiment, the absorbance value of the experimental group was lower than of the control group at 24,48,72 h, and the difference was statistically significant(P<0.05). In the Transwell cell migration experiment, the number of the experimental group and the control group was(207.67±7.23) and(369.00±41.22), respectively, with statistically significant differences(P<0.05). The results of flow cytometry detection of cell cycle showed that the G1,S and G2 phases of the experimental group were smaller than those of the control group, with cell cycle arrest. The results of cell apoptosis showed that the apoptosis rates of the experimental group and the control group were(22.61±5.70)% and(4.52±2.12)% respectively, with statistically significant differences(P<0.05). Conclusion The miR-30 a-5 p/MTA1 axis may have a certain inhibitory effect on the pathogenesis of laryngeal squamous cell carcinoma.