〔Abstract〕 Objective To investigate the role of nuclear factor of activated T cells 5(NFAT5) in inflammol/lation cascade in sepsis induced kidney injury. Methods Cultured mouse kidney medullar collecting duct cells 3 were stimulated by lipopolysaccharide(LPS)(10 ng/ml) for 12 hours with or without siRNA transfected. qRT-PCR was used to detect the transcription level of cytokine tumor necrosis factor α(TNFα) and chemokinemonocyte chemotactic protein-1(MCP-1) respectively, ELISA was applied to measure the protein of secreted in the culture medium. Chromatin immol/lunoprecipitation(ChIP) was used to detect whether NFAT5 bind the promotor of TNFα and MCP-1 respectively. Results After LPS stimulation for 12 hours,compared with the control group,the secretion of protein and transcription level of mRNA of TNFα and MCP-1 were upregulated( P<0. 05),however,they did not increase after transfection of NFAT5 siRNA. Western blot was difficult to measure the expression of NFAT5 isolated from the nuclear after LPS stimulation for 12 hours,however,NFAT5 was detected binding to the promotor of TNFαand MCP-1 respectively by ChIP. Conclusion NFAT5 plays a role in sepsis induced acute kidney injury by mediating inflammolation,specifically speaking,by controlling the transcription of cytokine TNFα and chemokine MCP-1 in LPS stimulated mouse cell line iM CD3.