〔Abstract〕 Objective To investigate the effect and mechanism of insulin-like growth factor binding protein 2(IGFBP2) on insulin-like growth factor-1(IGF-1) induced proliferation, migration and invasion of hepatocellular carcinoma(HCC)cell line of HepG2 cells. Methods 15.6, 31.2, 62.5, 125, 250 ng/ml IGFBP2 and 50 ng/ml IGF-1 were used to stimulate HepG2 cells. Cell proliferation was detected by CCK-8 method, and cell migration and invasion were detected by transwell method. Western blot was used to detect the activation and expression of intracellular signaling pathway-related proteins. Results The combination of IGFBP2 and IGF-1 could promote the proliferation, migration and invasion of HepG2 cells compared with the effect of IGF-1. The combination of IGFBP2 and IGF-1 increased phosphorylation levels of IGF1 R, Akt and ERK in HepG2 cells without significant changes in total expression, and the protein expression of early growth response gene 1(EGR1) also increased. Conclusion IGFBP2 enhances IGF-1 induced proliferation, migration and invasion of HepG2 cells by activating Akt and ERK signaling pathways.