〔Abstract〕 Objective To identify two clinical isolate strains ofEggerthella lenta. Methods Two strains isolated from positive blood anaerobic culture were selected.The isolates were identified by MALDI-TOF MS and gram staining.Drug sensitivity test was performed by agar dilution method.At the same time,genomic DNA of the strains was extracted,16 S rRNA universal primer was used for PCR amplification,and the product was sequenced after recovery and purification,which was homologous with the sequences of the known bacteria in GeneBank database. Results The isolate was identified asEggerthella lentaby MALDI-TOF MS and 16 S rRNA gene assay.Drug sensitivity tests showed that the bacteria was sensitive to penicillin,piperacillin-tazobactam,ampicillin-sulbactam,imipenem,meropenem,metronidazole and clindamycin. Conclusion MALDI-TOF MS and 16 S rRNA detection can be used for identification of rare bacteria such asEggerthella lenta.