〔Abstract〕 Objective To investigate the effects of miR-122-5 p on epithelial-mesenchymal transformation(EMT) in human ovarian cancer SKOV-3 cells, and to elucidate its possible mechanism. Methods Human ovarian cancer SKOV-3 cells were culturedin vitroand transfected with miR-122-5 p mimic and negative control(mimic NC). Then the cells were divided into blank control group(Blank), mimic NC group, and miR-122-5 p mimic group. The expression of miR-122-5 p and ADAM10 mRNA was detected by real-time fluorescence quantitative PCR(qRT-PCR). Western blot was used to detect the expression of ADAM10 protein. Bioinformatics predicted the target matching of mir-122-5 p and ADAM10, and dual luciferase reporter system was used to verify the target relationship. In addition, miR-122-5 p mimic and ADAM10-overexpressed plasmid(pcDNA-ADAM10) were respectively or simultaneously transfected into SKOV-3 cells, then the cells were divided into blank control group(Blank), miR-122-5 p mimic group, ADAM10 overexpression group(pc-ADAM10) and miR-122-5 p mimic+pc-ADAM10 group. Transwell was used to detect the migration and invasion ability of SKOV-3 cells. Western blot was used to detect the expression of Notch signaling pathway related proteins Notch1, HES1 and HEY1, EMT related proteins Snail, E-cadherin and N-cadherin. Results Compared with mimic NC group, the expression of miR-122-5 p of SKOV-3 cells in miR-122-5 p mimic group increased(P<0.01), indicating that miR-122-5 p mimic was stably transfected into SKOV-3 cells. Compared with mimic NC group, the expression of ADAM10 mRNA and protein of SKOV-3 cells in miR-122-5 p mimic group was lower(P<0.01). ADAM10 was confirmed as a target gene of miR-122-5 p by the dual luciferase reporter system. Compared with Blank group, the migration and invasion of SKOV-3 cells in miR-122-5 p mimic group decreased(t=6.004,P<0.05;t=7.289,P<0.01), while that of pc-ADAM10 group increased(t=13.181,P<0.001;t=11.504,P<0.01); the expression of Notch, HES1, HEY1, Snail and N-cadherin proteins were suppressed(P<0.05;P<0.01), while that of pc-ADAM10 group decreased(P<0.01). Compared with pc-ADAM10 group, miR-122-5 p mimic+pc-ADAM10 group could alleviate the EMT promoting effect of pc-ADAM10, and down-regulate the Notch signaling pathway activity. Conclusion miR-122-5 p inhibits the EMT of human ovarian cancer SKOV-3 cells by blocking Notch signaling pathway, which may be related to miR-122-5 p targeting inhibition of ADAM10 expression.