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Objective By doping Cu2+with mesoporous silica, a copper silicate nano platform with chemical kinetic effect was prepared, which can catalyze hydrogen peroxide to produce strong oxidative hydroxyl radicals, realize the oxidative damage of cancer cells and lead to cancer cells death.Methods By hydrothermal method, mesoporous silica was etched by copper ion to prepare copper silicate nanoparticles.The morphology, crystal structure, porosity, specific surface area and other physical and chemical properties of the nanoparticles were characterized by transmission electron microscope, X-ray diffraction and nitrogen adsorption desorption curve.Secondly, the biocompatibility of mesoporous silica without copper ions was verified by cell experiments.At the same time, the number of cytotoxic hydroxyl radicals produced by the copper silicate nanoparticles in cancer cells was evaluated by cell experiments,and the therapeutic effect of the nanoparticles on cancer cells was further evaluated.Results TEM images showed that mesoporous silica and copper silicate nanoparticles showed spherical morphology with good monodispersity.The average hydration particle sizes were 85.9 nm and 204.4 nm,respectively.The results of nitrogen adsorption desorption curve showed that the specific surface area of mesoporous silica and copper silicate nanoparticles were 26.8 m2/g and 5.36 m2/g,respectively,and the pore size of the nanoparticles was 18.9 nm and 1.66 nm,respectively.Subsequently,through cell experiments,we knowed that mesoporous silica nanoparticles had excellent biocompatibility,while copper silicate nanoparticles showed a strong inhibition on cancer cells.Conclusion In this study,copper silicate nanoparticles were successfully prepared and achieved good antitumor performance,indicating that they have a good prospect of chemokinetic therapy.

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Objective To observe the effects of metformin(MET) on the MIF,CD74 and podocalyxin(PCX) expression in renal tissues of type 2 diabetic mellitus(T2 DM) rats,and explore its possible renoprotective mechanisms. Methods 10 SD rats served as normal control group(NC,n=10), 30 type 2 diabetes SD rats induced by high fat diet and streptozotocin were randomly divided into DM group, MET group and glibenclamide(GLY) group. 8 weeks later, fasting blood glucose(FBG), hemoglobin A1 c(HbA1 c),blood urea nitrogen(BUN), urine albumin/creatinine(UACR), MIF/creatinine(UMCR), CD74/creatinine(UCCR), PCX/creatinine(UPCR), and MIF,CD74, PCX mRNA and relative protein expression in renal tissue were measured. Results (1) The FBG, HbA1 c, BUN, UACR, UMCR, UCCR, UPCR and the mRNA and protein expression of MIF and CD74 in renal tissue in MET group and GLY group were significantly lower than those in DM group,but higher than those in NC group(P<0.05). The expression of PCX in renal tissue was significantly higher than that in DM group, which was lower than that in NC group(P<0.05).(2) Compared with GLY group, UACR, UMCR, UCCR,UPCR and the expression of MIF and CD74 mRNA and protein in renal tissue in MET group decreased significantly, and the expression of PCX was significantly increased. There was no significant difference in FBG and HbA1 c levels between two groups. Conclusion MET exerts a reno-protective effect of type 2 diabetic rats, which may be related to its inhibition of the MIF-CD74 axis-mediated inflammatory cascade.

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Objecitve To investigate the preventive effect of ETOH from Asparagus officinalis L. and its different purified componentson carbon tetrachloride(CCl4) induced acute liver injury in mice. Methods Effects of ETOH and its components as petroleum ether extract(PE), ethyl acetate extract(EE), butanol extract(BE) were evaluated by CCl4induced acute liver injury model in mice. Levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bile acid(TBA) and Malondialdehyde(MDA),superoxide dismutase(SOD) in liver tissue as well as the pathology of HE were detected to find out the pharmacodynamic components, with which were further separated and evaluated. Results Levels of serum AST, ALT, TBA, MDA and SOD improved in the group of ETOH and BE(P<0.05), and the effect of BE was better than ETOH. Components of 30% ethanol eluate of butanol extract(BEA), 50% ethanol eluate of butanol extract(BEB), 70% ethanol eluate of butanol extract(BEC) and 90% ethanol eluate of butanol extract(BED) were further purified by BE. Pharmacodynamic evaluation showed that BEA improved the levels of AST, ALT, TBA in serum, and improved MDA and SOD in liver tissue(P<0.05). Content of phenolic acid and flavonoids in BEA was higher compared to that in BE. Conclusion Components named with ETOH, BE and BEA of Oufeng Asparagus officinalis L. can prevent CCl4induced acute liver injury, and the best pharmacodynamic components is BEA, which may be related to the enrichment of flavonoids and phenolic acids.

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Objective To prepare nanoparticles containing connective tissue growth factor with poly(L-lactic acid)(PLLA). Methods The connective tissue growth factor-loaded PLLA nanoparticles were prepared by double emulsion method. The characteristics of the nanoparticles were determined, and the morphology of the nanoparticles was observed. Finally, the release behavior of the nanoparticles was examinedin vitro. Results The average diameter of the prepared connective tissue growth factor-loaded PLLA nanoparticles was(376.5±18.2) nm, the incorporation efficiency and drug loading were(68.62±1.34) % and(34.26±0.73) μg/mg, respectively. The nanoparticles showed good spherical morphology without aggregation or adhesion. The release profile consisted of an initial fast release in the first 12 h and a more continuous slow release for 30 d. Conclusion The connective tissue growth factor-loaded PLLA nanoparticles prepared in this study are ideal nanoparticles to be applied on dental implant abutment surface.

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Objective To explore a new second-generation mammalian target of rapamycin(mTOR) inhibitor 9-(6-amino-3-pyridyl)-1-[3-(trifluoromethyl) phenyl] benzo[H]-1,6-naphthyridine-2(1 H)-one(Torin2) on the biological behavior of human papillary thyroid carcinoma(PTC) cells, so as to provide a new theoretical basis for clinical targeted treatment of PTC. Methods Human PTC cell line TPC-1 and human normal thyroid cell line Nthy-ori-3 were treated with different concentrations of Torin2. MTT colorimetry was used to detect cell proliferation, cell cycle and apoptosis were analyzed by flow cytometry, and cell migration was analyzed by scratching healing experiment. Results (1) Compared with that before administration, no significant change was observed in the human normal thyroid Nthy-ori-3 cell line;(2) different concentrations Torin2(100, 200, 400 nmol/L)after acting on TPC-1 cells 24, 48 and 72 h, cell proliferation inhibition rate gradually increased, and there was significant difference(P<0.001).And with the extension of action time(24,48,72 h) and the increase of concentration, the inhibition effect on cell proliferation was enhanced;(3)different concentrations Torin2(100, 200, 400 nmol/L)after acting on TPC-1 cells 24 and 48 h, the proportion of G1phase cells gradually increased, while the proportion of S-phase cells gradually decreased, and there was significant difference(P<0.001), indicating significant time and concentration dependence;(4) different concentrations Torin2(100, 200, 400 nmol/L)after acting on TPC-1 cells 24 and 48 h, the apoptosis rate increased significantly and showed a concentration-dependent effect, and there was significant difference(P<0.001);(5) different concentrations Torin2(100, 200, 400 nmol/L)at each concentration could inhibit the migration of TPC-1 cells, and with the increase of the concentration, the inhibition ability was stronger, and there was significant difference(P<0.001). Conclusion Torin2 can significantly inhibit the proliferation and migration of human PTC TPC-1 cells, block cell cycle, induce apoptosis, and enhance its anti-tumor effect with the increase of Torin2 concentration and the prolongation of action time.

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Objective To study the pathological mechanism of ornidazole inducing asthenospermia and its influences on spermatic mitochondrial function in rats. Methods Forty male SD rats were randomly divided into control group, low-dose, medium-dose and high-dose ornidazole group, 10 cases in each group. All the ornidazole groups were given 200, 400 and 800 mg/(kg·d) ornidazole for gavage, while control group was given the same amount of sodium carboxymethyl cellulose solution for 4 weeks. The general conditions in gavage process in each group were observed. At 30 minutes after the last administration, decapitation was conducted to collect unilateral testis and epididymis so as to calculate visceral indexes. The concentration and vitality of sperm were detected. And some epididymis and testis were preserved for light microscopy and electron microscopy. The other unilateral testes were applied to make tissue homogenate for detecting the activities and levels of acid phosphatase(ACP), alkaline phosphatase(ALP), lactate dehydrogenase(LDH), succinate dehydrogenase(SDH), superoxide dismutase(SOD) and malondialdehyde(MDA). The membrane potential changes of sperm mitochondria were detected by JC-1 staining. Results Compared with control group, body mass gain, testis, visceral indexes, sperm concentration, survival rate and activity of epididymis decreased after ornidazole treatment, showing concentration-dependence(P<0.05). Compared with control group, tissue morphology of testis and epididymis was damaged after ornidazole treatment. The higher the ornidazole dose was, the more severe the damage was. Compared with control group, activities of ACP, ALP, LDH, SDH and SOD decreased after ornidazole treatment, while MDA level increased, showing concentration-dependence(P<0.05). Compared with control group, ultrastructure of sperm mitochondria was damaged to different degrees under electron microscope after ornidazole treatment, and normal ratio of membrane potential also decreased. The changes in mitochondrial damage and normal ratio of membrane potential were the most significant after high-dose ornidazole treatment(P<0.05). Conclusion Ornidazole may destroy mitochondria structure by regulating energy metabolism and oxidative stress, thus leading to reproductive toxicity.

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Objective To investigate the effects of gene interference with miR-105 on stem cell-like characteristics, malignant proliferation and invasion of non-small cell lung cancer cells. Methods RT-PCR was used to detect the expression level of miR-105 in lung tissues and adjacent tissues of 33 patients with lung cancer. After transfection of miR-105 inhibitor into A549 cells, the cells were randomly divided into three groups: Control group, inhibitor-NC group and miR-105 inhibitor group for follow-up experiments. The expression level of miR-105 was detected by RT-PCR. The pellet formation rate and pellet formation diameter were measured by pellet formation experiment. Western blot was used to detect the expression levels of SOX2 and OCT4 proteins. The number of CD133 positive cells was determined by flow separation. Cell proliferation was detected by clone formation assay. Transwell was used to detect cell invasion; Western blot was used to detect VEGF protein expression level. Results The results showed that the expression level of miR-105 in paracancer tissues was lower than that in cancer tissues. Compared with the Control group, miR-105 inhibitor group of miR-105 expression level decreased(P<0.05), the cells into a ball rate, into a ball diameter decreased(P<0.05), SOX2, OCT4 protein levels(P<0.05)and CD133 positive cells were lower(P<0.05), clone formation rate was lower(P<0.05), invasive cells were lower(P<0.05), VEGF protein levels were lower(P<0.05). Conclusion Gene interference with miR-105 can well inhibit stem cell-like characteristics, malignant proliferation and invasion of non-small cell lung cancer cells.

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Objective To investigate the effects of Cyp4 a14 gene, Malondialdehyde(MDA) and the related inflammatory factors on intestinal mucosal oxidative stress in mice. Methods Male mice of Cyp4 a14+/+and Cyp4 a14-/-were selected and randomly divided into 4 groups: Cyp4 a14+/+control group, Cyp4 a14+/+DSS group, Cyp4 a14-/-control group, Cyp4 a14-/-DSS group. Both control groups were given cleaning water to drink for 6 days, and both DSS groups were given 3.0% DSS solution to drink for 6 days. Colon inflammation was comprehensive evaluated by changes in constitution/quantity, disease activity index(DAI), colonic length and HE staining. The mRNA levels of pro-inflammation cytokines(IL-1β and TNF-α) were quantified by Real-time PCR. The concentration of MDA in serum was measured by biochemical method to evaluate the degree of oxidative stress. Results There was no signs of enteritis in the two control groups, Cyp4 a14-/-DSS group showed mild enteritis, while Cyp4 a14+/+DSS group showed severe enteritis. The expressions of IL-1β and TNF-α in the colon increased both in Cyp4 a14+/+DSS group and Cyp4 a14-/-DSS group, and Cyp4 a14+/+DSS group increased more significant than Cyp4 a14-/-DSS group. The serum concentration of MDA in Cyp4 a14-/-control group decreased compared with that in Cyp4 a14+/+control group. Compared with the two control groups, serum MDA concentrations both increased in the Cyp4 a14+/+DSS group and the Cyp4 a14-/-DSS group, especially in the Cyp4 a14+/+DSS group, There were differences among the four groups(F=573.233,P<0.001). Conclusion Lipid peroxides MDA decreases in colon tissues of mouse knockout of Cyp4 a14 gene, and the sensitivity to DSS-induced colitis decreases, suggesting that Cyp4 a14 gene may be a promoter of oxidative stress in colon tissues of mice.

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Objective To investigate the effect of Curcumin(Cur) on the proliferation, apoptosis and cycle of human gastric cancer cell line SGC-7901 and its molecular mechanism.Methods SGC-7901 cells were treated with different concentration of Cur(0,10,20,40 μmol/L).3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was used to detect the changes in cell proliferation at different time points(24,48,72 h).Hoechst 33258 fluorescence staining was used to observe cell status of apoptosis.Flow cytometry was used to detect apoptosis and cell cycle.However,the changes of related protein expression in PI3K/p53 signaling pathway were detected by Western blot.Results MTT results showed that the proliferation of SGC-7901 cells was inhibited by Cur in concentration-dependent manners.The results of cell fluorescence staining showed that Cur could promote the apoptosis of SGC-7901 cells,and the bright blue fluorescence intensity of cells reduced.The results of flow cytometry showed that compared with the control group,the apoptosis rate increased(P<0.05,P<0.01) and G0/G1 phase cell was blocked in the group with different concentrations of Cur groups(P<0.05).Western blot results showed that the protein level of PI3K and the phosphorylation level of Akt in cells of different concentrations reduced compared with the control group(P<0.01),but the expression levels of p21 and p53 increased(P<0.05,P<0.01).Conclusion Cur inhibits the proliferation of SGC-7901 cell,induce G0/G1 phase arrest and promote apoptosis,which may be closely related to the regulation of PI3K/p53 signaling pathways.

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Objective To investigate the effect of Apelin-13 regulation of VEGF/PI3 K/Akt signaling on femoral fracture healing. Methods Forty male SD rats were randomly divided into sham group, Model group, Apelin-13 low-dose group[Apelin-13 L, 25 μg/(kg·d)] and Apelin-13 high-dose group[apelin-13 H, 75 μg/(kg·d)], and 10 rats in each group. The model of femoral fracture was established and immediately treated with appropriate drugs for 4 weeks. Micro CT and X-ray were used to detect bone microscopic parameters and callus diameter. HE staining was used to observe bone tissue pathological changes. ELISA was used to detect the expression levels of serum bone markers BALP, PINP and osteoclast TRACP-5 b, CTX. qRT-PCR was used to detect VEGFA mRNA expression levels in callus tissues, and Western blot was used to detect the expression levels of related proteins in the VEGF/PI3 K/Akt signaling pathway in callus. Results Compared with the sham group, the Model group had severe pathological damage of femur and decreased femur bone density, tissue mineral density, bone mass fraction and X-ray score. BALP and PINP levels in serum decreased, while TRACP-5 b and CTX levels increased, and VEGFA mRNA, VEGFA, VEGFR2, PI3 K p85 and p-Akt expression levels in callus decreased. Compared with the Model group, the pathological injury of the femur in the Apelin-13 H group improved. Bone mineral density, tissue mineral density, bone volume fraction, X-ray score and callus diameter all increased. BALP and PINP levels in serum increased(P<0.05), while TRACP-5 b and CTX levels decreased, and VEGFA mRNA, VEGFA, VEGFR2, PI3 K p85 and p-Akt expression levels in callus increased. There was no significant difference between Apelin-13 L group and Model group(P>0.05). Conclusion Exogenous Apelin-13 can promote femoral fracture healing in rats, and its mechanism may be related to the activation of the VEGF/PI3 K/Akt signaling pathway.

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Objective To investigate the effects of miR-122-5 p on epithelial-mesenchymal transformation(EMT) in human ovarian cancer SKOV-3 cells, and to elucidate its possible mechanism. Methods Human ovarian cancer SKOV-3 cells were culturedin vitroand transfected with miR-122-5 p mimic and negative control(mimic NC). Then the cells were divided into blank control group(Blank), mimic NC group, and miR-122-5 p mimic group. The expression of miR-122-5 p and ADAM10 mRNA was detected by real-time fluorescence quantitative PCR(qRT-PCR). Western blot was used to detect the expression of ADAM10 protein. Bioinformatics predicted the target matching of mir-122-5 p and ADAM10, and dual luciferase reporter system was used to verify the target relationship. In addition, miR-122-5 p mimic and ADAM10-overexpressed plasmid(pcDNA-ADAM10) were respectively or simultaneously transfected into SKOV-3 cells, then the cells were divided into blank control group(Blank), miR-122-5 p mimic group, ADAM10 overexpression group(pc-ADAM10) and miR-122-5 p mimic+pc-ADAM10 group. Transwell was used to detect the migration and invasion ability of SKOV-3 cells. Western blot was used to detect the expression of Notch signaling pathway related proteins Notch1, HES1 and HEY1, EMT related proteins Snail, E-cadherin and N-cadherin. Results Compared with mimic NC group, the expression of miR-122-5 p of SKOV-3 cells in miR-122-5 p mimic group increased(P<0.01), indicating that miR-122-5 p mimic was stably transfected into SKOV-3 cells. Compared with mimic NC group, the expression of ADAM10 mRNA and protein of SKOV-3 cells in miR-122-5 p mimic group was lower(P<0.01). ADAM10 was confirmed as a target gene of miR-122-5 p by the dual luciferase reporter system. Compared with Blank group, the migration and invasion of SKOV-3 cells in miR-122-5 p mimic group decreased(t=6.004,P<0.05;t=7.289,P<0.01), while that of pc-ADAM10 group increased(t=13.181,P<0.001;t=11.504,P<0.01); the expression of Notch, HES1, HEY1, Snail and N-cadherin proteins were suppressed(P<0.05;P<0.01), while that of pc-ADAM10 group decreased(P<0.01). Compared with pc-ADAM10 group, miR-122-5 p mimic+pc-ADAM10 group could alleviate the EMT promoting effect of pc-ADAM10, and down-regulate the Notch signaling pathway activity. Conclusion miR-122-5 p inhibits the EMT of human ovarian cancer SKOV-3 cells by blocking Notch signaling pathway, which may be related to miR-122-5 p targeting inhibition of ADAM10 expression.

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Objective To explore the molecular mechanism of fasudil-modified macrophages in the treatment of experimental allergic encephalomyelitis(EAE). Methods C57 BL/6 mice were induced by myelin oligodendrocyte glycoprotein(MOG) to establish an active immune EAE model. Peritoneal macrophages were prepared after 9 days of immunization and the mice were either unable to be treated with or without fasuldil for 72 h(control group and experimental group). Macrophages from the control group and the experimental group were transfused into EAE mice models, with each mouse containing 5×107cells. After treatment for 10 days,the changes of clinical behavior and body weight of mice in the control group and experimental group were analyzed. Macrophages in the two groups were isolated from the abdominal cavity and the ratio of M1 and M2 macrophages in the two groups was analyzed by flow cytometry. The expression of macrophage markers in M1 and M2 was analyzed by fluorescence quantitative PCR. The levels of interleukin-10(IL-10) and interleukin-12(IL-12) were analyzed by enzyme-linked immunosorbent assay(ELISA). Results The clinical symptom score of the experimental group was significantly lower than that of the control group(F=6.135,P<0.001). Compared with the control group, the weight loss of mice in the experimental group was less, and the difference was statistically significant(F=3.105,P=0.011). Compared with the control group, the M2-labeled arginase(Arg-1), YM-1, FIZZ and IL-10 cytokine levels in the observation group increased significantly, while the M1-labeled nitric oxide synthase(iNOS) and IL-12 cytokine levels decreased significantly(t=7.091,P<0.001;t=11.094,P<0.001;t=6.182,P<0.001;t=3.942,P<0.001;t=4.132,P<0.001;t=3.198,P<0.001). Compared with the control group, the proportion of macrophages in F4/80-CD206 increased significantly, while the proportion of macrophages in F4/80-CD16/32 decreased significantly(t=10.618,P<0.001;t=12.105,P<0.001). Conclusion Fasudil-modified macrophages can significantly improve the clinical symptoms of EAE mice, and its mechanism is mainly through improving the polarity of macrophages.

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Objective To investigate the clinical significance and biological functions of family with sequence similarity 50 member A(FAM50 A) in hepatocellular carcinoma(HCC). Methods FAM50 A mRNA and protein were examined respectively by Real-time PCR and Western blot in 45 cases of HCC tissues and paired adjacent tissues. The expression and location were analyzed by HPA database. Oncomine and GEPIA databases were used to analyze the expression difference of FAM50 A in HCC tissues and normal liver tissues. HCC dataset was collected from The Cancer Genome Atlas database. The correlation between expression level of FAM50 A and clinicopathological features of HCC was analyed by SPSS 21.0. Relationship between expression level of FAM50 A and prognosis was analyzed by Kaplan Meier Plotter. The MethHC database was used to analyze the methylation level of FAM50 A promoter region. Moreover, the String database was used to analyze the network of proteins interacting with FAM50 A and GSEA was used to predict the possible signal pathways of FAM50 A in HCC. Results For normal tissue, the hightest expression level of FAM50 A was found in skeletal muscle, and the expression of FAM50 A in the liver ranked 37 th. For tumor tissue, the hightest expression level of FAM50 A was found in ovarian cancer, and the expression of FAM50 A in liver cancer ranked 9 th. FAM50 A was mainly located in cytoplasm, membrance, nuclear in normal liver tissue and FAM50 A was mainly located in nuclear in HCC tissue. Real-time PCR showed that FAM50 A mRNA expression level was higher in tumor tissues compared with adjacent tissues, and the difference was statistically significant(P<0.001). Western blot showed that FAM50 A protein expression level was higher in tumor tissues compared with adjacent tissues, and the difference was statistically significant(P<0.05). Oncomineand GEPIA databases showed that FAM50 A mRNA expression in HCC tissue was higher than in normal liver tissue, and the difference was statistically significant(P<0.05). FAM50 A mRNA correlated with gender(P=0.006), cirrhosis(P=0.001) and vascular tumor thrombus(P=0.010), not with age, tumor size, pathological stage, Alpha-fetoprotein(AFP) level, degree of differentiation or distant metastasis(P>0.05). Patients in FAM50 A high expression group had higher overall mortality and poorer prognosis than those in low expression group, the difference was statistically significant(P<0.000 1), and it might be an oncogene. The methylation level of FAM50 A promoter region in HCC tissue was lower than that in normal liver tissue, and the difference was statistically significant(P<0.005). The proteins interacting with FAM50 A included SF3 B3, ATP6 AP1, NSFL1 C, etc. The samples with high expression of FAM50 A mRNA showed enrichment of genes in the pathways of MAPK signaling pathway, Wnt signaling pathway, Notch signaling pathway, etc.(P<0.05). Conclusion FAM50 A is high expression in HCC tissues. FAM50 A expression level is associated with the malignant degree and poor prognosis of HCC. Moreover, it may act as an oncogene and can be used as an indicator for diagnosis and prognosis evaluation of HCC patients.

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Objective To analyze the effec of dexmedetomidine(Dex) postprocessing in alleviating oxidative stress injury and nuclear factor erythroid 2 related factor 2(Nrf2)/antioxidant response element(ARE) signaling pathways in cerebral ischemia-reperfusion rats. Methods 30 SD rats were randomly divided into sham operation group, model group and Dex group, 10 cases in each group. The modified Longa method was referred to make middle cerebral artery occlusion model in model group and Dex group. The Dex group was intraperitoneally injected with 100 μg/kg Dex(10 mg/L) at immediate reperfusion. After 24 h of reperfusion, The neurological defect score was performed. The cerebral infarction volume was detected by triphenyl tetrazolium chloride(TTC) method. The levels of superoxide dismutase(SOD), malondialdehyde(MDA) and nitric oxide(NO) in brain tissues were determined by ELISA. RT-PCR and Western blot were performed to detect expression of B-cell lymphoma 2(Bcl-2), Bcl-2 associated X protein(Bax), cleaved caspase-3, transcription factor E2 associated factor 2(Nrf2), hemeoxygenase-1(HO-1) and quinone oxidoreductase 1(NQO-1). The brain tissues injury was detected by HE staining and TUNEL staining. Results Compared with sham operation group, neurological defect score score, apoptosis rate, MDA and NO level in model group and Dex group increased(P<0.05), while SOD activity in brain tissue decreased(P<0.05), the expressions of Nrf2, HO-1, NQO-1, cleaved Caspase-3/Bcl-2 and Bax/Bcl-2 were up-regulated(P<0.05). Compared with model group, neurological defect score, cerebral infarction volume, apoptosis rate, MDA and NO level decreased in Dex group(P<0.05), SOD activity in brain tissue increased(P<0.05), and the expressions of Nrf2, HO-1 and NQO-1 were up-regulated(P<0.05), and the expressions of cleaved Caspase-3/Bcl-2 and Bax/Bcl-2 were down-regulated(P<0.05). HE staining showed that pathological changes of brain tissues reduced. Conclusion Dex postprocessing can reduce the oxidative stress injury in CIRI rats, which may be related to the activation of Nrf2/ARE signaling pathway.

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Objective A human erythroleukemia(HEL) cell xenograft model was established using zebrafish embryos to provide a model for studying in vivo behavior and practical drug testing of hematological tumors.Methods Divide the HEL cells into a blank group and a CM-DiI group. The CM-DiI group was labeled with a live cell tracer.Wild-type zebrafish were cultivated and reproduced. After embryogenesis, the embryos were incubated at 28 ℃ for 48 h until the membrane was broken. The zebrafish were divided into blank control group, PBS experimental group,and HEL experimental group. Cells of CM-DiI group were transplanted into zebrafish yolk sac of HEL experimental group by microinjection,and PBS experimental group was injected with PBS. The three groups of zebrafish were cultured in an incubator at 34 ℃,and whether the model was established successfully was identified through in vivo and in vitro proliferation experiments and in vivo fluorescence observation,the successfully identified zebrafish were exposed to cytarabine drug,divided into 50,100,200 nmol/L group and model control group,the proliferation and migration of leukemia cells in xenograft tumor models after drug exposure in each group were observed. Results The proliferation status of HEL cells in the CM-Dil group and the blank group was similar,and the difference was not statistically significant(P>0.05). The proliferation of HEL cells in zebrafish was confirmed by in vitro and in vivo proliferation experiments; The number of zebrafish in the HEL experimental group was lower than that in the blank control group and PBS injection group(P<0.05). Compared with the model control group,the proliferation of the three concentrations of cytarabine drug exposure group was lower(P<0.05). Conclusion Human acute erythroleukemia HEL cells can be proliferated after transplantation into zebrafish embryos,and the proliferative activity can be interfered by cytarabine,confirming the successful establishment of the xenograft model.

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Objective To investigate the differential expression of long noncoding RNA(LncRNA) gene in the regeneration of skull defects in young sheep and its effects in improving skull defects. Methods Twelve 1-month-old small-tailed Han sheep were divided into the primary bone tissue group and the skull defect group, with 6 in each group. High-throughput sequencing was used to detect the expression changes of LncRNA in the two groups of bone tissues, and Real-time PCR was used to verify the changes in the expression of sequencing LncRNA. The serum alkaline phosphatase(ALP) and osteocalcin(OCN) were detected by ELISA. The protein expression levels of Wnt and β-catenin were detected by Western blot. Results The expressions of LncRNA ODSM and LncRNA UCA1 were up-regulated, while the expressions of LncRNA H19 and LncRNA MEG3 were down-regulated in the new bone tissue; the content of ALP and OCN in serum of the new bone group increased; the expression of Wnt/β protein in bone increased compared with the original bone tissue group. Conclusion LncRNA ODSM and UCA1 may regulate Wnt/β signaling pathway and participate in the regeneration of skull tissue at the defect site.

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Objective To explore the pathogenesis of diabetic peripheral neuropathy based on the mitochondrial pathway regulated by SREBP-2/Lipin1. Methods 60 male SD rats, 20 rats as control group, 40 rats were given high-sugar high-fat diet combined with intraperitoneal injection of streptozotocin(STZ) induced diabetic mellitus(DM) rat model, feeding for 6 weeks, DPN model was established successfully when the rat tail sciatic nerve conduction veloeity(SNCV)<30 m/s. Selected ten rats in the normal control group and 10 rats in the DPN model group, the content of reactive oxygen species(ROS), SREBP-2 and Lipin1 protein expression in the sciatic nerve were detected. In addition, 30 diabetic peripheral neuropathy(DPN) rats were randomly divided into empty vector(NC) group, SREBP-2-siRNA(si-SREBP-2) group, Lipin1 over expression(LV-lipin1) group.The si-SREBP-2 group were injected with SREBP-2-siRNA through the tail vein, the LV-lipin1 and LV-control groups were respectively injected with Lipin1 lentivirus vector and empty lentivirus vector through the tail vein. The SREBP-2 and Lipin1 protein expression in the sciatic nerve were detected. After 8 weeks, the fasting plasma glucose and SNCV were observed. The mitochondrial membrane potential, ROS, Bcl-2, Bax, Cyto C in sciatic nerve were detected. Results Compared with the control group, ROS content and SREBP-2 protein expression were significantly increased in the DPN group, while the Lipin1 protein expression was significantly decreased in the DPN group. After regulated the expression of SREBP-2 and Lipin1, compared with the NC group, the SREBP-2 protein expression was significantly decreased while the Lipin1 protein expression was significantly increased in the DPN group. Fasting blood glucose significantly decreased, the SNCV significantly increased. Mitochondrial membrane potential significantly increased; The content of ROS significantly reduced. The protein expressions of Cyto C and Bcl-2 were significantly increased, while the protein expression of Bax was significantly decreased. Conclusion The SREBP-2 and Lipin1 expression are abnormal in the sciatic nerve of DPN rats, regulating SREBP-2 and Lipin1 can improve the sciatic neuropathy of DPN rats, indicating that the pathogenesis of DPN is related to the mitochondrial pathway regulated by srebp-2 and Lipin1.

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Objective To construct a human derived pEGFP-C1-p38γ expression plasmid and observe its expression of inflammatory factors in alcohol-induced L0-2 cells and its effect on cell proliferation and apoptosis. Methods RNA was extracted from L0-2 cells and reversely transcribed into cDNA. At the same time, the primers were diluted to 10 μmol/L and the rest were used as storage fluid. P38γ was amplified by PCR technology and identified. The DNA gel recovery kit AxyPrep was used for the purification and recovery of PCR products. Then the PCRproducts and carriers were recovered by enzyme digestion. After enzyme digestion and identification, they were sent to sequencing for identification and sequencing. The constructed plasmids were transfected into alcohol-induced L0-2 cells of human mononuclear macrophage cell line. The effects on cell proliferation and apoptosis were detected by MTT assay and flow cytometry, and the expressions of inflammatory cytokines IL-6 and TNF-α in alcohol-induced L0-2 cells were detected by Western blot. Results Sequencing results showed that pEGFP-C1-p38γ eukaryotic expression plasmid was successfully constructed. The results of MTT assay showed that 24 h later, the cell proliferation rate of the p38γ overexpressed group(0.42±0.08) % was significantly lower than that of the control group(0.60±0.03)%. The results of flow cytometry showed that the apoptosis rate of p38γ overexpressed cells group was(17.46±1.52) %, significantly higher than that in the control group(13.18±1.34) %(P<0.05). Western blot result showed that the expression of IL-6 and TNF-α in L0-2 cells transfected with pEGFP-C1-p38γ plasmid was higher than that in the control group. Conclusion P38γ can significantly inhibit the proliferation and promote the apoptosis of alcohol-induced L0-2 liver cells. P38γ promotes the expression of inflammatory cytokines IL-6 and TNF-α in alcohol-induced L0-2 cells.

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Objective To explore the effect of Arctigenin on renal ischemia-reperfusion injury model in aged rats. Methods The model of renal ischemia-reperfusion injury in elderly rats was established, and the rats were randomly divided into 6 groups by random number table method: Sham group, Arctigenin group(ACR), renal ischemia-reperfusion model group(RIRI), RIRI+ACR(10 mg/kg) group, RIRI+ACR(20 mg/kg) group and RIRI+ACR(50 mg/kg) group for follow-up experiments. Kit was used to detect Scr and BUN levels. HE staining was used to detect pathological damage of renal tissue. Apoptosis was detected by TUNEL staining. The levels of inflammatory cytokines TNF-α, IL-6 and IL-8 were detected by ELISA. The expression levels of KIM-1, NGAL, cleaved caspase-3, cleaved caspase-9, PI3 K, p-PI3 K, Akt and p-Akt were detected by Western blot. After the PI3 K/Akt pathway inhibitor LY294002 was added, the rats were randomly divided into 5 groups: Sham group, RIRI group, RIRI+ACR(50 mg/kg) group, RIRI+LY294002 group and RIRI+ACR+LY294002 group. Western blot was used to detect the expression levels of PI3 K, p-pi3 k, Akt and p-akt in the rats in each group. The kit was used to detect the levels of Scr and BUN, and ELISA was used to detect the levels of TNF-α, IL-6 and IL-8. Results The results showed that compared with RIRI group, RIRI+ACR(20, 50 mg/kg) group of Scr and BUN levels in rats decreased obviously, obviously improved pathological damage degree, KIM-1, NGAL protein levels significantly decreased, apoptotic cells significantly reduced, cleaved caspase-3, cleaved caspase-9 protein levels significantly decreased, the TNF alpha, IL-6, IL-8 levels significantly decreased, p-PI3 K/PI3 K, p-Akt/Akt ratio increased significantly. Conclusion Arctigenin has protective effect on renal ischemia-reperfusion injury in aged rats.

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Objective To investigate the effect of sevoflurane on cerebral ischemia-reperfusion injury(CIRI) and the role of silent information regulator 1(SIRT1) as well as autophagy. Methods SD rats were divided into sham group, middle cerebral artery ischemia(MCAO) model group, sevoflurane(0.6%, 1.2%, 2.4%) treatment groups, rats were pretreated with different dose of sevoflurane, middle cerebral artery occlusion was used to establish model and neurological score was evaluated, the infarct volume was measured by TTC staining, and the brain water content was assessed, HE staining was used to detect the pathological change, cell apoptosis rate was measured by TUNEL assay, the contents of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-10(IL-10) were measured by ELISA assay, SIRT1 gene expression was detected by RT-PCR, the protein levels of SIRT1,LC3 and p62 were detected by Western blot. Autophagy inhibitor 3-MA and SIRT1 siRNA were used to treat rats,and their effects on CIRI injury were examined. Results Compared with sham group,the MCAO model rats appeared neurological deficit,infarct volume rate increased,brain water content increased,significant pathological changes and apoptosis in the cerebral cortex appeared,inflammation increased,SIRT1 gene and protein expression increased,level of autophagy increased; sevoflurane pretreatment could alleviate the cerebral ischemiareperfusion injury,meanwhile,the SIRT1 expression and autophagy levels were further elevated. The autophagy inhibition of 3-MA and SIRT1 silence could reverse the protective effect of sevoflurane in cerebral ischemia-reperfusion,and decrease the level of autophagy. Conclusion Sevoflurane can attenuate cerebral ischemia-reperfusion injury in rats by inducing SIRT1-dependent autophagy.

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Objective To investigate the expression of Fibulin-2 in hepatocellular carcinoma(HCC), and to explore the effects of Fibulin-2 on the biological changes of HepG2 hepatoma cells and the expression of matrix metalloproteinase-2(MMP-2), matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor(VEGF).Methods The expression of Fibulin-2 in 20 cases of HCC carcinoma and paracancerous tissues was detected by Western blot and qRT-PCR.In the cell experiment,Fibulin-2 overexpression and silencing treatment were divided into common hepatocellular carcinoma group(common group),silence-NC group(Si-NC group),Fibulin-2 silencing group(Si group),overexpression-NC group and Fibulin-2 overexpression group.MTT colorimetry,cell scratch test,Transwell assay and flow cytometry were used to detect cell proliferation,migration,invasion and apoptosis rate.Western blot was used to detect the expression of MMP-2,MMP-9 and VEGF after Fibulin-2 overexpression or silencing.Results The results of qRT-PCR and WB experiments showed that the expression of Fibulin-2 in hepatocellular carcinoma tissues was significantly lower than that in corresponding paracancerous tissues at the translation and transcriptional levels(P<0.05).The expression levels of MMP-2,MMP-9 and VEGF in Si group were higher than those in common group(P<0 05).The migration,invasion and proliferation ability of HepG2 hepatoma cells were enhanced and the apoptosis rate was decreased(P<0 05).Compared with the normal group,the expression levels of MMP-2,MMP-9 and VEGF in the overexpression group were decreased,and the migration,invasion and proliferation ability of liver cancer cells were inhibited and the apoptosis was increased(P<0.05).Conclusion The expression of Fibulin-2 in HCC tissues was significantly lower than that in paracancerous tissues.Overexpression of Fibulin-2 could inhibit the malignant characteristics of HCC cells,while silencing Fibulin-2 could enhance the progression of HCC cells.Fibulin-2 may affect the biological characteristics of hepatocellular carcinoma cells by regulating the expression levels of MMP-2,MMP-9 and VEGF.

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Objective To investigate the effect of natural product isoliquiritigenin(ILTG) on the sleep of C57 mice and the possible effects of brain areas. Methods The control group C57 BL/6 J background mice were fed with saline for 7 days, and the administration group C57 BL/6 J background mice were fed with isoliquiritigenin for 7 days(32.4 mg/kg). The mice's sleep-wakefulness processes were recorded and analyzed by electroencephalogrphy(EEG), electromyogram(EMG) and software; The water maze experiment was used to investigate whether ILTG damaged the memory ability of mice; c-fos staining was performed on the whole brain to find which regions the drugs acted on. Results(1) On the 7 th day of intragastric administration, the total amount of sleep in the administration group increased compared to the control group, non-rapid eye movement sleep(NREM) increased, there was no significant difference in REM sleep; sleep latency was shortened, and brain increased energy spectrum;(2) In the experiment of water maze, there was no significant difference in the time to find the plateau area(quadrant 5) between the control group and the drug group, and there was no significant difference in the residence time in the plateau area;(3) The c-fos staining showed that the brain regions activated by ILTG were ventrolateral preoptic area(VLPO), ventromedial preoptic nucleus(VMPO), and lower fornix(SFO). Conclusion ILTG can increase the amount of NREM sleep in C57 mice and increase the power spectrum of brain waves during NREM. The brain regions activated by ILTG's sleep-promoting effects are VLPO, VMPO, and SFO brain regions. Moreover, isoliquiritigenin has no neurotoxicity and will not damage the cognition and memory of mice.

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To investigate and compare the drug resistance, virulence genes, capsular genes and ST type of Hypermucoviscous Klebsiella pneumoniae(HMKP) in Anhui regoin in Septermber 2017 and September 2018 to provide evidences for the formulation of HMKP prevention. 137 strains were isolated and identified by slime test. The minimum inhibitory concentration of antimicrobial drugs was determined by agar dilution method. Polymerase chain reaction was carried out to detect their virulence gene and serotype. Molecular typing was performed with multi-locus sequence typing. The carrying rate between the HMKP virulence gene rmpA or rmpA2 positive group and the negative group was compared. 137 strains were mainly from sputum specimens, and the isolation rate was relatively high in aged 70-79. The resistance rate of the strain to gentamicin and levofloxacin had increased. The aerobactin carrying rate was the highest in both years. K2 were the major capsular serotypes and ST65 were the major clones in 2017, K1 were the major capsular and ST23 were the major clones in 2018. In addition, the HMKP virulence gene rmpA positive group was more likely to carry rmpA2 and infect people aged 70-79 than the negative group. The HMKP virulence gene rmpA2 positive group was more likely to carry the kfu and infect people aged 70-79 than the negative group. The strains should be defined based on their phenotypes and genes for timely treatment.

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Objective To explore the effects of pre-pregnancy BMI and gestational weight gain on the measure-ment indexes of obesity in preschool children, and provide evidence for weight management of pregnant women. Methods 1 769 pregnant women who underwent prenatal examination in maternal and child health institutions were selected as the study subjects. “Maternal and Child Health record form during pregnancy and delivery period” and the hospital electronic medical system were used to collect information such as pre-pregnancy body mass index(BMI) and gestational weight gain(GWG). The “Health and behavior record of Preschool Children” was used for childhood follow-up to collect information such as physical activity of feeding patterns of preschool children. Physical examination on collected physical measurement data such as upper arm circumference, upper arm skinfold thickness, abdominal wall skinfold thickness, height and weight, and described the distribution of related characteristics and the effects of pre-pregnancy BMI and weight gain during pregnancy on the measurement index of obesity in preschool children. Results The number of pregnant women with excessive GWG are 770, accounting for 50.7%. There were statistically significant differences in Pre-pregnancy BMI(χ2=46.08,P<0.01), annual family income(χ2=15.07), number of pregnancies(χ2=12.22) and different pregnancy attitudes(χ2=19.05) among different GWG groups. After adjusted confounding factors such as education and education, pregnant women with excessive GWG was positively correlated with upper arm circumference of preschool children(β=0.091, 95%CI: 0.118 to 0.505), upper arm skin fold thickness(β=0.086, 95%CI: 0.217 to 1.067), abdominal wall skinfold thickness(β=0.059, 95%CI: 0.014 to 0.938) and age-specific BMI(β=0.150, 95%CI: 0.205 to 0.456). The pre-pregnancy low BMI group and upper arm circumference of preschool children(β=-0.182, 95%CI:-0.896 to-0.522), upper arm skinfold thickness(β=-0.138, 95%CI:-1.586 to-0.758), abdominal wall skinfold thickness(β=-0.118, 95%CI:-1.563 to-0.638), age-specific BMIZ score(β=-0.182, 95%CI:-0.588 to-0.342) were negatively correlated. Conclusion Excessive GWG is a risk factor for obesity in preschool children, and lower pre-pregnancy BMI is a protective factor for obesity in preschool children.

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Objective To explore the differences in clinical characteristics and risk factors of patients with psoriasis vulgaris of different genders. Methods The cases were investigated by the questionnaire star. The data were statistically processed and analyzed by SPSSAU. Results There were 375 valid questionnaires. The proportion of men with psoriasis was greater(67.73%) than that of women. Women had earlier first-onset-age than men(P=0.004) and had a longer course of disease(P=0.021). More than 85% of male and female patients had onset before the age of 40. The initial site of skin lesions was mostly scalp and extremities. The patients mainly felt itching and dryness,and most of the lesions occurred in winter while the least occurred in summer. Female patients had a greater proportion of family history(P=0.025). The family history of both sexes was mostly at first grade.Associated diseases were mainly hypertension and dyslipidemia. Male patients were more educated than females(P=0.002),and there was no significant difference in other clinical characteristics. The proportion of men who were overweight or obesity,drinking and smoking was higher(P<0.01). Conclusion There are more men than women in psoriasis vulgaris patients. Females have an earlier first-onset age and long course of disease. Most patients get sick before the age of 40. There are many cases in winter,and itching and dryness are the main feelings.Associated diseases are mainly hypertension and dyslipidemia. Overweight or obesity,alcohol consumption,and smoking are more important risk factors for male patients.

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Objective To study the pathogen types and drug resistance of hospital acquired pneumonia(HAP) in the respiratory intensive care unit(RICU), and to provide a reference for the empirical anti-infection treatment of HAP. Methods The pathogen types of HAP patients in RICU ward were statistically analyzed, and their resistance to antimicrobial agents and risk factors of MDRO infection were analyzed. Results A total of 156 pathogenic bacteria were cultured from blood and sputum samples of lower respiratory tract of 81 HAP patients, among which 153 pathogenic bacteria were cultured from sputum. Among them, 99(63.46%) gram-negative bacteria, 7(4.49%) gram-positive bacteria and 50(32.05%) fungi were found. A total of 75 strains of MDRO(excluding duplicate strains), accounting for 48.08% of the total bacteria, including 72 strains of gram-negative bacteria(96.00%) and 3 strains of gram-positive bacteria(4.00%), were cultured from the blood or sputum of 48 patients(59.26%). There were significant differences in the days of stay at RICU, days of invasive mechanical ventilation, days of antibiotic use and days of PPI, indwelling central venous catheter, tracheotomy status between MDRO infection and non-MDRO infection(P<0.05). However, there was no significant difference in patients' age, complicated MODS, diabetes mellitus. Conclusion The MDRO infection rate in HAP patients is high, so the possibility of MDRO infection should be fully taken into account when choosing the initial anti-infection regimen, antimicrobial agents should be reasonably used and mechanical ventilation should be withdrawn in time to avoid the long-term use of PPI.

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Objective To analyze the difference in clinical stages for patients with diabetic retinopathy(DR) through monitoring the area of foveal avascular zone(FAZ) and retinal microvessel density(MVD) by using optical coherence tomography angiography(OCTA). Methods 232 patients(260 eyes) with DR were divided into four groups according to the international staging criteria. Specifically,the patients were respectively categorized as mild non-proliferative diabetic retinopathy(NPDR) group(63 eyes),moderate NPDR group(64 eyes),Severe NPDR group(66 eyes) and hyperplastic diabetic retinopathy(PDR) stage Ⅳ group(67 eyes). The healthy subjects(60 eyes) were selected as the control group. The macular FAZ and MVD were measured using OCTA. At the same time,the glycated hemoglobin(HbAlc) and fasting plasma glucose(FPG) of the subjects were tested. The changes of FAZ and MVD in the surface and deep retinal capillary layers,and the changes of HbAlc,FPG were statistically analyzed and compared between the different groups. The correlation between the changes in FAZ,MVD and the progression of DR,the correlation between changes in HbAlc,FPG and MVD were established respectively.Results OCTA examination showed that FAZ gradually increased,while MVD gradually decreased in patients with DR,and that no perfusion area appeared in these patients. The levels of Hb Alc and FPG also gradually increased in each group with the development of DR. Statistical analysis showed that the differences between the control group and diabetes groups were significant(P<0.05). The same difference was found between the diabetes groups. Correlation coefficient showed that the expression of FAZ and MVD in the surface and deep retinal capillary layers were positively correlated with the progression of diabetes(P<0.05). The same positive correlation was found between HbAlc,FPG and MVD. Conclusion OCTA examination may show the changes in the microcirculation disturbance of the macular retina in diabetic patients,which can be used as a clinical indicator of DR staging.

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Objective To investigate the changes and mechanisms of attention function lateralization in Parkinson's disease(PD). Methods The lateralized attention network test was used to compare the orientation, alertness and executive function of both cerebral hemispheres of 32 PD patients and 28 health controls(HCs) by calculating the mean response time(RT) and error rate(ERR). Results In the orientation network, the RT of the right field of vision of the HCs group was longer than that of the left, showing a statistically significant difference(t=4.751,P<0.000 1), while the RT of the left field of vision(67.63±43.59) ms of PD was longer than that of the right(32.02±52.99) ms, showing a statistically significant difference(t=2.923,P=0.006 3). In the alertness network, there was no significant difference in RT between the left and right visual fields in the HCs group, and the left visual field RT in PD group was negative, no alertness effect was observed. In the executive network, there was no significant difference in RT between the left and right field of HCs and PD. There was no significant difference in ERR between the two groups in the three networks. Conclusion The orientation function of PD patients showed the dominance of the right cerebral hemisphere, while the HCs showed the dominance of the left cerebral hemisphere, and the right cerebral alertness was impaired in the PD group, suggesting that the change of the dominant cerebral hemisphere of the attention function of PD patients might be related to the abnormal secretion of neurotransmitters in the left and right cerebral hemispheres.

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Objective To provide the scientific evidence for the prevention and cure of stroke by investigating its risk factors in residents of Hefei Silihe community.Methods A total of 2 682 permanent residents aged ≥40 years were included in this study via cluster sampling method.Carotid vessel color ultrasound examination was implemented in the high-risk group of stroke.Results 762 high-risk people were screened out,with a detection rate of 28.41%.In a descending order,the exposure rates of risk factors were 86.35% for hypertension,62.99%for dyslipidemia,48.29% for lack of exercise,34.38% for overweight or obesity,31.76% for family history of stroke,28.22% for diabetes,23.49% for smoking,and 3.94% for atrial fibrillation.The detection rate of male was higher than that of female in high risk group(37.92%,22.99%)(χ2= 67.970,P<0.001).Smoking rate in male was higher than that in female(χ2= 240.6,P<0.001).Exposure rate of risk factors of female stroke family history was higher than that of male(χ2= 7.16,P<0.05).There were 867 cases of high Hcy group in high-risk population(32.35%).The positive ratio of carotid atherosclerosis was 65.08%.Logistic regression analysis showed that lack of exercise and storke were significantly related(OR=33.675,95% CI: 22.061-49.239).Conclusion Exposure rate of risk factors for stroke in high-risk population in Hefei Silihe community is high.It is necessary to identify the high-risk group of stroke early.Intervene modifiable risk factors actively,standardize the diagnosis,and treatment of chronic diseases like hypertension and diabetes are benefit to reduce the prevalence of stroke.

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Objective To explore the epidemiological characteristics of influenza in Anhui province from 2017 to 2018, so as to provide evidences for the formulation of influenza prevention and control. Methods The data were collected from information system for China diseases control and prevention,including influenza-like illness(ILI) case report, pathogen surveillance report.SPSS 16.0 software was used for data analysis. Results The ILI number and average percentage of influenza-like cases(ILI %) in Anhui province from 2017 to 2018 were 283 827 and 4.42 % respectively. Both ILI and ILI % were highly seasonal, with a significant peak in January 2018. The proportion of ILI cases was highest in age group 0-4(59.74 %), whereas lowest in age group 60 and above(3.36 %). From 2017 to 2018, a total of 38 869 swab samples from ILI patients were collected and tested,from which 5,490 specimens were RNA positive, with a positive rate of 14.12 %. The positive rate varied among different months, and different age groups(P<0.001). The percentages of virus subtypes were significantly different among people in different months and age groups(χ2=7 092.0,P<0.001; χ2=879.1,P<0.001). There were positive corelations both between ILI %, positive rate of influenza and the ILI counts(r s =0.585,P<0.05;r s =0.691,P<0.05). Conclusion The percentage of ILI in children under the age of 5 is the highest, and the number of ILI cases in winter 2018 has surged compared to 2017. Different virus subtypes prevail alternatively from 2017 to 2018. The main subtypes of influenza virus are different among different age groups.

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Objective To understand the clinical characteristics and laboratory data of lung space-occupying lesion, and to improve the understanding and diagnostic ability of the nature of lung space-occupying lesion. Methods 190 patients with lung-occupying space lesion who have clear pathological results by tracheoscopic biopsy or CT guided percutaneous lung biopsy were divided into benign and malignant lesion group. Malignant lesion group were further divided according to pathological types. The clinical characteristics and laboratory data of all patients were recorded in detail, then analyzed and compared between the groups. Results (1) Among 190 patients, 37(19.47%) had benign lesions while 153(80.53%) had malignant lesions. There were significant differences in smoking, lung cancer and HSP90 between benign lesion group and malignant group. 92.77% of smoking patients with high three indicators of lung cancer or HSP90 were malignant lesions. However, there was no significant difference in age, course of disease, gender, family history, CRP, abnormal glycoprotein and D-dimer between benign lesion group and malignant lesion group.(2) In the malignant lesion group, adenocarcinoma was the most, accounting for 50.98%; squamous cell carcinoma was the second, accounting for 30.07%; small cell carcinoma was 17.65%, and other types accounted for 1.31%. There were significant differences in the course of disease, gender, family history, smoking, CRP and lung cancer among different types of lung cancer, but there was no significant difference in age, abnormal glycan protein, HSP90 and D-dimer. Conclusion For the patients with smoking, and high three indicators of lung canceror high HSP90, the lung-occupying space lesion are more likely to be malignant. Combined with the clinical characteristics and laboratory data, the malignant lesion can be further diagnosed.

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Objective To investigate the correlation between clinical physiological with the severity as well as the prognosis of idiopathic pulmonary fibrosis, and to analyze the value of quantitative analysis of CAD system for the prognosis of IPF. Methods 126 IPF patients were divided into survival group and non-survival group retrospectively according to their outcome during the follow-up. Data of clinical features and quantitative analysis parameters of imaging measured by CAD system were recorded and analyzed simultaneously. Disease progress was compared between the two groups and Cox regression models were used to identify the association between clinical features, quantitative analysis parameters of CAD system and all-cause mortality. Results Indicators of lung function including FVC, DLCO and TLC significantly increased in survival group compared with non-survival group(P<0.05). Imaging changes analyzed with CAD system in the patients with IPF showed that volume ratio of reticulation honeycombing and total ILD lesions were significantly higher than the patients in survival group(P<0.05). The multivariate Cox regression model showed that FVC,TLC,DLCO,volume ratio of reticulation, volume ratio of HC, volume ratio of total ILD lesion and deterioration within 6 months could elevate mortality risk of the patients with IPF. Conclusion Baseline pulmonary function parameters can predict mortality risk. The quantitative analysis of CAD system is an accurate, sensitive, objective and repeatable evaluation for the degree of IPF disease.