〔Abstract〕 Objective To investigate the WNT ligands regulated by TCF7 L2 in gastric cancer cells and their functions on the proliferation and metastasis of gastric cancer cells. Methods qPCR and PCR array methods were used to detect the expression of TCF7 L2 and WNT7 b genes in gastric cancer cell lines and clinical specimens. 200 ng/ml recombinant human WNT7 b protein was used to treat gastric cancer cell lines SGC7901 and N87. qPCR, Western blot and dual-luciferase reporter gene detection system were used to detect TCF7 L2 and β-catenin/TCF binding activity, respectively. 2 μg/ml actinomycin D treatment was used to analyze the effect of WNT7 b on TCF7 L2 mRNA stability in N87 cells. CCK-8 kit, wound healing and transwell methods were used to analyze the abilities of proliferation and migration of gastric cancer cells. Results By analyzing PCR array data of SGC7901/TCF7 L2, N87/shTCF7 L2 and their corresponding control cell lines, it was found that WNT7 b was the most remarkable in the influenced WNT ligands by TCF7 L2 expression. The detection of 17 gastric cancer specimens showed that the transcription levels of TCF7 L2 and WNT7 b in gastric cancer tissues were both higher than those in normal adjacent tissues, and there should be a positive correlation between the expressions of TCF7 L2 and WNT7 b. TCF7 L2 transcription levels increased after the 24 h treatment of WNT7 b protein on N87 cells(P<0.001). Meanwhile, TCF7 L2 mRNA stability was enhanced(P<0.05), and TCF7 L2 protein levels and the β-catenin/TCF binding activity were increased(P<0.05). Moreover, it was demonstrated that WNT7 b and TCF7 L2 enhanced the proliferation and migration abilities of gastric cancer cells(P<0.05) through the cytology experiments. Conclution The expression levels of TCF7 L2 and WNT7 b in gastric cancer tissues are higher than those in normal control. There is a mutual expression enhancement between WNT7 b and TCF7 L2, which plays a role in regulating the proliferation and migration of gastric cancer cells together.