〔Abstract〕 Objective To investigate the regulatory effect of YAP gene on the expression of TGF-α and EGFR in cervical cancer cells infected with HPV. Methods Immunohistochemistry and Western blot were used to detect the expression of YAP, TGF-α and EGFR in HPV-16(+) Ca Ski, HPV-18(+) C4-1 human cervical cancer cells and HPV(-) C33 a human cervical cancer cells. After intervention with the YAP inhibitor Vertepofin, it affected the proliferation of Ca Ski and C4-1 cells and C33 a,as well as the expression of mRNA and protein in YAP,TGF-α,and EGFR. Results The expressions of YAP,TGF-α and EGFR in HPV-16( +) Ca Ski and HPV-18( +)C4-1 human cervical cancer cells were higher than that of HPV(-) C33 a cells; after Vertepofin intervention,the cell proliferation rate decreased. At the same time that YAP was inhibited,the expression of mRNA and protein in TGF-α and EGFR also appeared to be inhibited. Conclusion The Hippo/YAP signaling pathway for EGFR and TGF-α expression in HPV-infected cervical cancer cells can play a positive regulatory role.