〔Abstract〕 Objective To study the effect of high fat diet on metabolism and body fat of offspring mice in different periods(pregnancy and lactation) by feeding maternal mice with high fat diet. Methods ICR pregnant mice were divided into control group(C) and high-fat group(H) randomly,after delivery, each group(mentioned above) was subdivided into two groups: C and H. Therefore, four groups were formed: CC group, CH group, HC group and HH group. After weaning on the 21 st day, the offspring mice of all groups were fed with common feed until the 8 th week(end of adolescence). Then the other three groups were fed with high-fat diet until the 15 th week except the CC group. Results The blood glucose and cholesterol of placenta and fetus in group H were higher than those in group C. At the 8 th week, the serum total cholesterol level of HH group was higher than that of CH group. At the 15 th week, the body weight, body fat percentage and insulin level of CH group were higher than those of the control group, and the fasting blood glucose of HH group was higher than that of CC group. Conclusion High fat diet during pregnancy will lead to the disorder of glucose and lipid metabolism in offspring mice, and the offspring are more likely to be obesity and metabolic disorder after being induced by high-fat diet in adulthood if their mother mice are fed with high-fat diet during lactation.