〔Abstract〕 Objective To investigate the effect of miR-664 b-5 p on the biological behavior of esophageal cancer cells EC9706 and TE-1. Methods Quantitative real-time PCR(qRT-PCR) was used to determine the expression of miR-664 b-5 p after transfected with miR-664 b-5 p mimic or inhibitor in esophageal cancer cells EC9706 and TE-1; Cell proliferation was assessed by CCK-8 and colony formation; Migration and invasion of esophageal cancer cells were observed by the transwell chamber assay; The expression of apoptosis-related proteins Bax and Bcl-2 were detected by Western blot. Results After the transfection of miR-664 b-5 p mimic, the expression level of miR-664 b-5 p increased in esophageal cancer cells EC9706 and TE-1(P<0.05), while the transfection of miR-664 b-5 p inhibitor was the opposite(P<0.05); The results of cell proliferation assay showed that the ability of the cell proliferation and the number of colony formation decreased in the miR-664 b-5 p mimic group compared with the mimic NC group(P<0.05), and the ability of the cell proliferation and the number of colony formation in the miR-664 b-5 p inhibitor group was higher than the inhibitor NC group(P<0.05); The results of transwell assay showed that the number of migration cells and invasive cells of esophageal cancer in the miR-664 b-5 p mimic group was lower than that of the mimic NC group(P<0.05), while the number of migration cells and invasive cells in the miR-664 b-5 p inhibitor group was higher than that of the inhibitor NC group(P<0.05); The results of Western blot showed that the expression of Bax increased and Bcl-2 reduced in the miR-664 b-5 p mimic group(P<0.05), but the expression of Bax reduced and Bcl-2 increased in the miR-664 b-5 p inhibitor group(P<0.05). Conclusion miR-664 b-5 p can inhibit cell proliferation,migration and invasion,and promote the apoptosis of esophageal cancer cells EC9706 and TE-1, which provides the foundation to further study the molecular mechanism of miR-664 b-5 p in Kazakh patients with esophageal squamous cell carcinoma.