〔Abstract〕 Objective To sequence the T cell receptor(TCR) of patients with Graves ophthalmopathy(TAO) before and after the treatment of intravenous injection of methylprednisolone, and to further explore the immunologic mechanism of the treatment of Tao by intravenous injection of methylprednisolone, so as to provide theoretical basis for the new immunologic treatment. Methods Six patients with TAO were collected from the outpatient department of the First Affiliated Hospital of Xiʼan Jiaotong University. The peripheral blood was collected at three time points of diagnosis, the end of the first shock treatment and the end of the third shock treatment. The peripheral blood mononuclear cells( PBMCs) were extracted and RNA was extracted for TCR immunohistochemistry library sequencing,followed by high-throughput sequencing data mining analysis. Results After three hormone shocks,there was no significant difference in TCR diversity index,VJ family migration and VJ family frequency among the improved TAO patients. Conversely,the high frequency VJ family showed clonal expansion in worsening TAO patients. TAO remission was linked with the decrease of Th17 cells. Conclusion The mechanism of intravenous shock therapy of TAO by methylprednisolone is the reestablishment of immune balance rather than the elimination of autoreactive cloning.