〔Abstract〕 Objective To investigate the effect of hydrogen sulfide(H2S) on proliferation, migration and relaxation of rat basilar artery(BAs) smooth muscle cells(VSMC) and the relationship between H2S and vascular endothelial growth factor receptor 2(VEGFR2). Methods Primary cultured rat BAs VSMC. Specifically, immunofluorescence was used to identify the expression of VEGFR2in BAs VSMC. Cell scratch test was used to observe the change of migration ability of BAs VSMC. CCK-8 method was applied to detect the effect of H2S on the proliferation of BAs VSMC. Cell relaxation test was engaged to detect the relaxation ability of H2S on VSMC. Results The results of cell scratch and value-added experiments showed that, compared with the control group, exogenous H2S donor NaHS(100 μmol/L) could increase the proliferation and migration ability of BAs VSMC and promote VSMC relaxation(P<0.05). In addition, 10 ng/ml VEGF could also enhance the migration, proliferation and relaxation capacity of BAs VSMC, which was similar to the effect of NaHS. On the other hand, pretreatment with 10-5mol/L VEGFR2blocker SU5416 could reduce NaHS cell proliferation, migration and relaxation. Conclusion H2S can promote the proliferation, migration and cell relaxation of VSMC, which may be related to the stimulation of VEGFR2.