〔Abstract〕 Objective To investigate the effects of imperatorin on hippocampal injury and atpase activity in rats after cerebral ischemia and reperfusion. Methods The rat model of cerebral ischemia-reperfusion was established,and the rats were randomly divided into 6 groups: healthy control group, model group, imperatorin 25,50,100 mg/kg group and nimodipine group for follow-up experiments. Y maze experiment detected the learning and memory function of rat brain. HE staining was used to detect pathological damage of brain tissue in rats. Apoptosis of rat neurons was detected by TUNEL staining. The contents of total superoxide dismutase(SOD), malondialdehyde(MDA) and glutathione peroxidase(GSH) were detected by the kit. The activity of lactate dehydrogenase(LDH), Na+-K+-ATP and Ca2+-Mg2+-ATP were detected by the kit. Survivin, Caspase-3 and Caspase-9 were detected by Western blot. Results Compared with model group, healthy controls and extraneous imperatorin 50, 100 mg/kg and nim horizon group armed into a significant rise in number of incubation period(P<0.05), the extraneous arms, starting arm into the number, the other arm into number significantly reduced(P<0.05), significantly improved pathological damage degree and the apoptosis positive cells were significantly reduced, Survivin protein level significantly increased(P<0.05), Caspase 3, Caspase-9 proteins significantly reduced(P<0.05), SOD and GSH contents significantly increased(P<0.05), MDA content significantly decreased(P<0.05), LDH activity significantly decreased(P<0.05), Na+-K+-ATP, Ca2+-Mg2+-ATP activity significantly increased(P<0.05). Conclusion Imperatorin has a protective effect on cerebral ischemia and reperfusion in rats.