〔Abstract〕 Objective To investigate the influence on cisplatin resistant of ovarian cancer regulating the expression of miR-214-3 p and analyze the effects of miR-214-3 p on early growth response protein 1(EGR1). Methods Lipofection transfection technology was used for transient inhibition or increasing of miR-214-3 p expression. Cell viability and the half concentration(IC50) of DDP were determined by CCK-8 method. Cell apoptosis was detected by flow cytometry. The mRNA expressions of miR-214-3 p and EGR1, the protein levels of EGR1 and xeroderma pigmentosum(XPD) were measured by using Real-time quantitantive PCR(qRT-PCR) and Western blot, respectively. Results miR-214-3 p in C13 K cells was down-regulated than those in OV2008 cells(P<0.001). After 48 h of transfection, qRT-PCR results revealed that the expression of miR-214-3 p was higher in mimics groups C13 K cells(22.42±4.27) compared to the blank control C13 K cells(0.33±0.16)(P<0.001). The expression of miR-214-3 p in inhibitor groups OV2008 cells(0.11±0.09) was lower than that of blank control OV2008 cells(7.92±2.22)(P<0.001). The decreasing cell proliferation and promoted apoptosis in C13 K cells after transfected with miR-214-3 p mimics was detected(P<0.01), while the increasing of cell proliferation and decreasing of apoptosis in OV2008 cells after transfected with miR-214-3 p inhibitor was detected in the same time(P<0.01). The IC50of DDP in mimics group C13 K cells was decreased, while was promoted in inhibitor group OV2008 cells after transfected(P<0.01). With increased miR-214-3 p expression by mimics transfected, the mRNA and protein expressions of EGR1 greatly increased, and the protein levels of XPD decreased in C13 K cells. By down-regulation of miR-214-3 p, the mRNA and protein expressions of EGR1 greatly decreased, and the protein levels of XPD increased in OV2008 cells. Conclusion miR-214-3 p plays the role of ovarian cancer cisplatin resistant. EGR1 protein is a possible downstream target of miR-214-3 p in ovarian cancer cells.