〔Abstract〕 Objective To investigate the relationship between vitamin D receptor(VDR) gene polymorphism and susceptibility to pulmonary tuberculosis( PTB) in adolescents,and to provide genetic theoretical basis for prevention and treatment of PTB in adolescents. Methods Peripheral venous blood was collected from 96 patients with pulmonary tuberculosis( as the case group) and 123 healthy controls( as the control group) in Hefei and Shannan.The genotypes of VDR( ApaI,BsmI,FokI,TaqI) were detected by PCR-RFLP,and Chi-square test was used to analyze the relationship between VDR gene polymorphism and TB susceptibility in adolescents from two regions. Results The polymorphism of BsmI locus was significantly different between the case group and the control group in Hefei and Shannan( Hefei: χ2= 19. 948,P<0. 001,Shannan: χ2= 38. 485,P<0. 001). The polymorphism of FokI locus was significantly different between the case group and the control group in Hefei and Shannan( Hefei: χ2=7. 231,P = 0. 027,Shannan: χ2= 21. 781,P<0. 001). But the polymorphisms of ApaI and TaqI locus showed no significant difference between the case group and the control group( P>0. 05). Conclusion VDR gene BsmI and FokI locus polymorphism are related to susceptibility to tuberculosis in adolescents,the Bb,bb,Ff and ff genotypes increase the susceptibility to tuberculosis in adolescents.