〔Abstract〕 Rat spleen cell suspension was prepared by common method. The expression levels of helper T cells(CD3+CD4+), cytotoxic T cells(CD3+CD8+), and B cells(CD45 R+) subsets tryptophan 2, 3-dioxygenase(TDO2) in rat spleen were detected by Cytomics FC500(FC500) and ImageStreamXMark Ⅱ(Mark Ⅱ) flow cytometry. The correlation and consistency of the results were observed respectively. The expression of TDO2in CD3+CD4+T cells, CD3+CD8+T cells and CD45 R+B cells were measured by two kinds of flow cytometry,and the correlation were well(R2>0.8). Bland-Altman method was used to analyze TDO2expression in CD3+CD4+T cells, CD3+CD8+T cells and CD45 R+B cells. The bias were-1.3%,-8.2% and 7.4%, respectively.TDO2were expressed in T and B cells of rat spleen. These two flow cytometries had good correlation and consistency in testing resualts. FC500 is easy and fast to operate. Mark-Ⅱ combines the high-throughput analysis of traditional flow cytometry with cell mor-phology analysis, which is highly accurate and can obtain specific cell pictures.