〔Abstract〕 Objective To explore the expression of adipocyte enhancer-binding protein 1(AEBP1) in glioblastoma(GBM) and its effect on the prognosis. Methods The expression of AEBP1 mRNA in GBM was analyzed by Oncomine database and GEPIA website. The GEPIA website and TCGA data were used to analyze the correlation between the expression level of AEBP1 mRNA and various clinicopathological features, as well as its influence on the prognosis of GBM. The expression of AEBP1 protein in glioma was further analyzed in the human protein atlas(HPA) database. Finally, the GO and KEGG analysis of AEBP1 and its co-expression genes were performed in WebGestalt website. Results All 7 data sets selected from Oncomine database and GEPIA website analysis showed that AEBP1 mRNA expression was up-regulated in GBM tissues, and the difference was statistically significant(P<0.01). The survival analysis of GEPIA website showed that the overall survival(OS) of patients with high AEBP1 mRNA expression reduced, and the difference was statistically significant(P=3.9×10-5). Further TCGA database analysis showed that AEBP1 mRNA expression level was correlated with IDH1 mutation(P=0.005), and it was an independent factor influencing the OS in patients with GBM(HR=0.563 1(0.390 2, 0.812 7),P=2.16×10-3). KEGG pathway enrichment analysis showed that AEBP1 and its co-expressed genes were mainly enriched in Hippo, NF-κB, TNF and other signaling pathways. Conclusion AEBP1 is highly expressed in GBM, and the high expression of AEBP1 mRNA is an independent prognostic factor for patients with GBM. Hippo, NF-κB, TNF and other signaling pathways may be the important pathway for AEBP1 overexpression to promote the occurrence and development of GBM. AEBP1 is a potential target for GBM patients.