〔Abstract〕 Objective Cancer-associated pancreatic stellate cells(CaPSCs) and normal pancreas-associated pancreatic stellate cells(NaPSCs) were selected as entry points to screen circRNA related proliferation and migration of pancreatic cancer cell line Panc-1. And to explore strategies for targeted therapy of pancreatic cancer. Methods CaPSCs1～5and NaPSCs1～5were isolated and cultured from pancreatic cancer tissues of 5 pancreatic cancer patients and normal pancreas tissues of 5 patients with benign pancreatic disease. After they were co-cultured with Panc-1, and then the proliferation ability of Panc-1 was tested by CCK-8 experiment. CaPSCs1, which had the strongest promoting effect on Panc-1 proliferation, and NaPSCs1, which had the weakest effect, were screened.And then RNA high-throughput sequencing was used to analyze the differential expression profiles of circRNA between the two kinds of cells,and qRT-PCR was used to verify the first three circRNAs with the most differential expression. At the same time,KEGG was used to enrich the biological functions and signaling pathways of differential circRNA,and microRNA target prediction software was used to predict differential circRNA downstream genes( microRNA). Results Compared with Na PSCs1,CaPSCs1group had 841 differential circRNAs( 453 down-regulated and 388 up-regulated),8 155 differential mRNAs( 4 226 down-regulated and 3 929 up-regulated),and 73 differential microRNA( 16 down-regulated and 57 up-regulated). Among them,the most significantly expressed circRNAs were chr7: 154954255 | 154998784,chr10: 12081472 | 12120267,and chr10: 76909966 | 77019099. The differences were statistically significant( P<0. 05). The corresponding target genes( microRNA) were hsa-miR-612,hsa-miR-1301-3 p and hsa-miR-4459. The major signaling pathways enriched by differential circRNA were the Toll-like receptor signaling pathway,the TNF signaling pathway,the T cell receptor signaling pathway,and the HIF-1 signaling pathway. The biological processes were mainly focused on cell-substrate junction and cell-substrate adherens junction etc. Conclusion CircRNAs are up-regulated in Ca PSCs: chr7: 154954255 | 154998784,chr10: 12081472 | 12120267,chr10: 76909966 | 77019099 and their downstream target genes( microRNAs) are likely to interact with PSCs to promote pancreatic cancer cell proliferation and migration through multiple signaling pathways.