〔Abstract〕 Objective To observe whether biochanin A(Bioch A) exerts protective effects on MPP+-induced PC12 cells apoptosis. Methods Effects of MPP+and Bioch A on PC12 cells viability were detected by MTT assay to screen out the MPP+-induced apoptosis dose and appropriate Bioch A dose. Conventional cultured PC12 cells were divided into: Control group, MPP+(1 μmol/L) group, Bioch A(1.25 μmol/L) group, Bioch A(2.5 μmol/L) group, Bioch A(5 μmol/L) group and Estradiol(E2)(0.1 μmol/L) group. Reactive oxygen species(ROS) was detected by DCFH-DA probe method. Apoptosis rate was detected by Annexin V-FITC/PI double staining. The expression levels of cleaved Caspase-3 and cleaved Caspase-9 were detected by Western blot method. Results Compared with the control group, ROS content increased in MPP+group. Compared with the MPP+group, ROS content decreased in Bioch A(2.5, 5 μmol/L) groups. Compared with the control group, the apoptosis rate and the levels of cleaved Caspase-3 and cleaved Caspase-9 increased in MPP+group. Compared with the MPP+group, the rate of apoptosis and the expression levels of cleaved Caspase-3 and cleaved Caspase-9 decreased in Bioch A(2.5, 5 μmol/L) groups. Conclusion Bioch A exerts protective effects on MPP+-induced PC12 cells apoptosis.