〔Abstract〕 Objective To investigate the effect of the aqueous extracts of mulberry on adipogenic differentiation of bone marrow mesenchymal stem cells(BMSCs) stimulated by H2O2. Methods BMSCs were isolated from the bone marrow of rats and identified by flow cytometry. The aqueous extracts of mulberry were prepared by vacuum freeze-drying. CCK-8 method was performed to detect the toxicity of mulberry aqueous extract(15, 30, 60 mg/L) to BMSCs. Oil red O staining was used to identify the lipid droplets. Immunofluorescence and Western blot were used to detect the expression of PPARγ and C/EBPα in BMSCs treated by the aqueous extracts of mulberry(15, 30, 60 mg/L). Results CCK-8 assay showed that the aqueous extracts of mulberry did not affect the growth of BMSCs(P>0.05). Oil red O staining showed that the aqueous extracts of mulberry inhibited the formation of lipid droplets in BMSCs induced by H2O2, showing a dose-effect relationship(P<0.01). The aqueous extracts of mulberry(60 mg/L) significantly inhibited the expression of PPARγ and C/EBPα in BMSCs stimulated by H2O2(P<0.05). Conclusion The aqueous extracts of mulberry can inhibit the adipogenic differentiation of BMSCs induced by H2O2trough PPARγ and C/EBPα signaling pathway.