〔Abstract〕 Objective To observe and analyze the effect of interfering VIRMA gene on the migration ability of gastric cancer cells, and preliminarily explore the mechanism. Methods The MGC803 and SGC7901 gastric cancer cells were transfected with lentivirus to interfere the expression of VIRMA. The cell proliferation in each group was detected by CCK-8. The migration ability of cells was detected by wound healing assay and Transwell assay. The changes of VIRMA and FN1 expression were detected by qRT-PCR and Western blot. Results qRT-PCR and Western blot showed that lentivirus effectively affected VIRMA gene expression in gastric cancer cells(P<0.05). Interfering VIRMA gene inhibited the proliferation of gastric cancer cells effectively(P<0.05). The 24 h wound healing distance of gastric cancer cells in the interference group was less, and the number of transmembrane cells was also less than that of the control group(P<0.05). After interfering with VIRMA gene, the expression of FN1 decreased(P<0.05). Conclusion Lentivious-mediated knockdown of VIRMA can inhibit the migration ability of gastric cancer MGC803 and SGC7901 cells, and the mechanism may be related to the regulation of FN1 expression.