〔Abstract〕 Objective To establish a TaqMan real-time fluorescent quantitative PCR of spotted fever group rickettsiae(SFGR). Methods According to theompAgene sequence ofRickettsia.japonica,the specific primers and probes were designed to develop the TaqMan-based Real-time PCR method. The specificity, sensitivity and repeatability of the established method were verified. The established TaqMan real-time PCR method was used to detect SPGR infection in 80 clinic blood samples collected in Anhui Province and compared with the nested PCR. Results The real-time quantitative PCR method for detecting SPGR was successfully established. The cyclic threshold of the standard curve and the template copy number showed a good linear relationship(r>0.99). This method had strong specificity, high sensitivity and good repeatability in the detection of the spotted heat rickettsial nucleic acid samples. Conclusion This study establishes a real-time quantitative PCR assay based on TaqMan probe to detect SPGR, which provides a rapid and effective technical means for rapid diagnosis in clinical laboratories.