〔Abstract〕 Objective To investigate whether the polymorphism of tryptophan hydroxylase 2(TPH2) gene is associated with erectile dysfunction(ED). Methods A total of 59 men diagnosed with ED and 151 healthy controls were recruited. International index of erectile function-5(IIEF-5) and the premature ejaculation diagnostic tool(PEDT) data were collected. All ED patients and healthy controls were genotyped for polymorphisms in the TPH2 gene. Allele and genotype frequencies of single nucleotide polymorphisms(SNPs) were compared between the patients and controls. Result The rs1007023 G allele frequency was significantly higher in ED patients than that in the control group(P=0.023). Conclusion The SNPs rs1007023 of the TPH2 gene seems to be associated with ED in males with the rs1007023 G allele or G/G+T/G allele in the Han population.