Found programs:
Authors:Wang Ziran; Ma Fan; Ding Pengsheng
Keywords:leucocidin;hepatocellular carcinoma;histone deacetylase 3
DOI:10.19405/j.cnki.issn1000-1492.2020.06.020
〔Abstract〕 Objective To study the effect of S component of panton-valentine leucocidin(LukS-PV) on proliferation of hepatocellular carcinoma(HCC) cells and its mechanism. Methods HepG2 and Huh7 cells were treated with LukS-PV at different concentrations(0.25,0.5,0.75,1 μmol/L).Cell proliferation was evaluated by CCK-8 method.Changes in overall acetylation level after LukS-PV treatment were detected by pan-acetylated antibody,and changes in protein expression profile of HepG2 cells after LukS-PV treatment were detected by quantitative proteomics.TCGA database was used to analyze the expression of histone deacetylase 3(HDAC3) in HCC and its prognostic correlation.RT-PCR and Western blot were used to verify the changes of HDAC3 mRNA and protein levels in HepG2 and Huh7 cells treated with different concentrations of LukS-PV.Changes in proliferation capacity of HCC cells were detected after HDAC3 knockdown with specific SiRNA. Results LukS-PV could decrease the proliferation ability of HCC cells in a time-dependent and concentration-dependent manner.LukS-PV could increase the acetylation level of HepG2 cells,and quantitative proteomics results showed that HDAC3 expression was down-regulated in HepG2 cells after LukS-PV treatment.TCGA data analysis showed that the expression level of HDAC3 mRNA in liver cancer tissues was higher than that in normal tissues,and the survival time of patients with high HDAC3 expression was lower than that of patients with low HDAC3 expression.The mRNA and protein levels of HDAC3 were down-regulated by LukS-PV in HepG2 and Huh7 cell lines.Knockdown of HDAC3 reduced the proliferation of HepG2 and Huh7 cells. Conclusion LukS-PV may inhibit the proliferation of HCC cells by down-regulating the expression of HDAC3,which provides a new research idea for the precise and targeted treatment of HCC.