〔Abstract〕 Objective To investigate whether advanced glycation end products(AGEs) have an effect on the release of endothelial microparticle(EMP). Methods Cultured human umbilical vein endothelial cells(HUVECs) were incubated with AGEs(100, 200, 400 μg/ml) for 12 or 24 h. Pretreatment with anti-RAGE antibody or reactive oxygen species(ROS) scavenger, HUVECs were incubated with 400 μg/ml AGEs for 24 h. EMP, RAGE and ROS were assessed. Results AGEs induced EMP releasing in a dose-dependent manner, not a time-dependent manner. Meanwhile, AGEs increased the levels of RAGE and ROS. Pretreatment with anti-RAGE antibody or antioxidant treatment could reverse the release of AGEs inducing EMP. Conclusion AGEs-RAGE interaction induced EMP generation, which was mediated by oxidative stress.