〔Abstract〕 Objective To observe the effect of mesencephalic astrocyte-derived neurotrophic factor(MANF) on experimental autoimmune uveitis(EAU) in rats. Methods Lewis rats were immunized with interphotoreceptor retinoid-binding peptide(IRBP) in order to generate EAU model. The experimental group was administered MANF on the 3 rd, 7 th and 11 th day after immunization, and the control group was administered phosphate buffered saline(PBS)as a positive control.The normal group was not treated. Caspi method was used to score the degree of inflammation. The rats were killed during the peak period of inflammation, and the retinal inflammatory reaction and histopathological changes of the three groups were detected by hematoxylin-eosin staining.The expressions of inter-leukin-17( IL-17) and interferon-gamma( INF-γ) were detected by real-time fluorescence quantitative PCR( RTqPCR). Results The inflammation began on the 3 rd day after immunization,and reached the peak on the 14 th day. The pathological and clinical manifestations on the 14 th day after immunization suggested that the ocular inflammation and pathological changes of the experimental group were significantly better than those of the control group. The expression levels of inflammatory factors IL-17 and INF-γ in the experimental group were significantly lower than those of the control group. Conclusion MANF vitreous cavity administration can reduce the inflammation of EAU in rats,and the decrease of the expressions of IL-17 and INF-γ may be a part of the effect,and the specific mechanisms need to be further studied.