〔Abstract〕 Objective The study was carried out to investigate the respective effects of insulin, glibenclamide and metformin on antenatal and postpartum lipid metabolism for GDM mice. Methods C57 BL/6 J mice were kept under observation for one week to acclimatize the new conditions,then mating. The pregnant mice were grouped as normal control group(N),GDM control group(G),GDM treated with insulin group(GI), GDM treated with glybenclamide group(GG), GDM treated with metformin group(GM).The mild amount of streptozotocin was used to stimulate C57 BL/6 J mice to develop GDM. For animals in the treated groups, treatment was initiated from GD11 to GD18. Results Three interventions respectively in gestational diabetes mellitu(GDM) maternal mice during pregnancy, and decrease total cholesterol(TC), triglyceride(TG), and low density lipoprotein-cholesterol(LDL-C) levels(P<0.05) while the high density lipoprotein-cholesterol(HDL-C) levels were increased significantly(P<0.05). Specifically, p-ACC levels were increased(P<0.05), while sterol regulatory element binding protein 2(SREBP2) were still reduced(P<0.05) respectively in murine livers from all the intervened groups compared with the GDM control. Conclusions Respective interventions of insulin, glibenclamide and metformin may significantly reduce blood lipid levels for maternal GDM mice during pregnancy.