〔Abstract〕 Objective To investigate the effect of TET1 gene on the proliferation and migration of HeLa cells. Methods The overexpression plasmid of TET1 was constructed by transcription activator-like effectors(TALE)-vp64 system and transfected into cervical cancer HeLa cells. The proliferation of HeLa cells was monitored by thiazolyl blue tetrazolium bromide(MTT), the migration ability of HeLa cells was detected by scratch test, and the invasion ability of HeLa cells was detected by Transwell. Results MTT assay showed that the proliferation ability of TET1 overexpressed cells was significantly lower than that of wild-types(P<0.05).The numbers of HeLa cells passed through the matrigel were(Clone 1, 21±5) and(Clone 2,22±6) in TET1 overexpresed cells, and(38±7) in wild-type HeLa group,the difference between the two group was statistically significant(P<0.05). The migration rates of HeLa cell in TET1 overexpressed(Clone 1, Clone 2) and the wild-type group at 24 h and 48 h were detected by scratch test, the latter was significantly higher than the former(P<0.01).TET1 overexpression could restrain the ability of proliferation, invasion and migration of HeLa cells. Conclusion Overexpression of TET1 gene can inhibit the biological behavior of cervical cancer cells.