〔Abstract〕 Objective To explore the effect of β-catenin regulating NF-κB pathway on distraction osteogenesis of premaxillary suture in rats, and to study the effect of β-catenin agonist on NF-κB and related osteogenic factors. Methods 45 SD rats were randomly divided into three groups: control group, maxillary expansion group, SB group(local injection at the time of maxillary expansion), control group and maxillary expansion group, all of which were injected with DMSO once a day. The rats in each group were killed on the 1 st, 4 th and 7 th day after the expansion. The width of the premaxillary suture was measured by Micro-CT, and the histological changes were observed by HE, Masson staining. IHC was used to observe the changes of related factors. Results During the experiment, the width of premaxillary suture in SB group was significantly wider than that in control group. The expression of p65 in SB group was lower than that in maxillary expansion group, the expression of β-catenin was stronger than that in maxillary expansion group, while the IOD value of osteocalatine(OCN), bone morphogenetic protein(BMP-2) in SB group was higher than that in maxillary expansion group(P<0.05). Conclusion β-catenin can down-regulate p65, and inhibit NF-kappa B pathway, and promote BMP-2, OCN expression, and accelerate the formation and calcification of new bone in premaxillary suture.