〔Abstract〕 Objective To investigate the effects of immunoglobulin gene superfamily 10(IGSF10) on proliferation, migration and invasion of lung adenocarcinoma cells. Methods Bioinformatics was applied to study the expression levels ofIGSF10in tumor tissues and normal tissues. Western blot and quantitative real-time PCR(qPCR) were used to detect the expression level ofIGSF10in lung adenocarcinoma cell lines and normal lung epithelial cells. Knockdown ofIGSF10, the effect of knockdown ofIGSF10on proliferation, migration and invasion of lung adenocarcinoma A549 cells was examined using cell counting kit-8(CCK-8), Transwell migration and invasion assay, scratch assay and plate cloning assay. The effects of knockdown ofIGSF10on the expression of invasion and migration-related genes in A549 cells were examined by Western blot and qPCR assays. Results IGSF10expression in lung adenocarcinoma tissues was lower than that in normal tissues(P<0.05).IGSF10expression in lung adenocarcinoma cell lines was lower than that in lung epithelial cells(P<0.05). Knockdown ofIGSF10promoted the ability of lung adenocarcinoma A549 cells to proliferate, proliferation, migration and invasion(P<0.05). Knockdown ofIGSF10promoted the expression of regulatory epithelial-mesenchymal transition marker Neural-cadherin(N-cadherin) and key transcription factors Snail family transcriptional repressor 1(Snail) and Snail family transcriptional repressor 2(Slug)(P<0.05) and inhibited the expression of Epithelial-cadherin(E-cadherin)(P<0.05). Conclusion Knockdown ofIGSF10may promote proliferation, migration and invasion of lung adenocarcinoma cells through activation of Snail, Slug/E-cadherin signaling axis, and this result may provide a potential new target for clinical diagnosis and treatment of lung adenocarcinoma.