〔Abstract〕 Objective To investigate the expression difference and potential clinical significance of 83 sequence similar member A(FAM83A) and β-catenin in cervical lesions. Methods UALCAN and GEPIA2.0 online databases were used to analyze the difference ofFAM83Aexpression in normal cervix and cervical squamous cell carcinoma(CSCC) and the relationship betweenFAM83Aexpression and the prognosis of CSCC patients, LinkedOmics database was used to analyzeFAM83AmRNA co-expression genes, and R language was used for KEGG enrichment analysis. Immunohistochemistry was used to detect FAM83A and β-catenin expression in 60 cases of normal cervix, 80 cases of low-grade squamous intraepithelial lesion(LSIL), 90 cases of high-grade squamous intraepithelial lesion(HSIL) and 70 cases of cervical squamous cell carcinoma(CSCC). The relationship between FAM83A, β-catenin expression and clinicopathological features and the correlation between FAM83A and β-catenin expression were analyzed. Results UALCAN database analysis showed thatFAM83Awas highly expressed in CSCC tissues, and GEPIA 2.0 database analysis suggested that those with highFAM83Aexpression had a poor prognosis. LinkedOmics database performing KEGG enrichment analysis suggested that expression ofFAM83Awas positively correlated with aberrant activation of Wnt/β-catenin signaling pathway. The expression rate of FAM83A in CSCC was higher than that in LSIL and normal cervical tissues(P<0.001), but there was no significant difference compared with HSIL(P=0.401); the expression of FAM83A was not correlated with age(P=0.231), but was significantly different from the correlation with differentiation(P=0.001) and clinical stage(P=0.038). The abnormal expression rate of β-catenin in CSCC was higher than that in LSIL and normal cervical tissues(P<0.001), but there was no significant difference compared with HSIL(P=0.734); the expression of β-catenin was not related to age(P=0.088), related to differentiation(P=0.001) and clinical stage(P<0.001), and FAM83A was positively correlated with β-catenin expression(P<0.05). Conclusion FAM83A and β-catenin are highly expressed in both HSIL and CSCC tissues, and there is a positive correlation between the expression of FAM83A and β-catenin. The high expression of FAM83A has some correlation with the prognosis of CSCC patients and can be used as a potential marker to determine the prognosis of CSCC.