〔Abstract〕 Objective To explore the role of long non-coding RNA XR＿378418(LncRNA XR＿378418) in the biological behavior of hepatic stellate cells line JS-1 and to probe the potential molecular mechanism of LncRNA XR＿378418 involved in liver fibrosis based on transcriptome sequencing. Methods In this study, the recombinant plasmid of pcDNA-LncRNA XR＿378418 and control plasmid pcDNA-NC were constructed and transfected into JS-1 cells respectively. Then, the expression level of LncRNA XR＿378418 was analyzed by quantitative real-time PCR(RT-qPCR). The effect of overexpression of LncRNA XR＿378418 on proliferation and migration of JS-1 cells were detected by cell counting kit-8 assay(CCK-8) and scratch assay, respectively. Finally, by high-throughput sequencing analysis, the effect of XR＿378418 on the transcriptomics of JS-1 cells was analyzed. Results RT-qPCR results showed that the expression level of LncRNA XR＿378418 in the overexpression group was significantly higher than that in the control group(P<0.05). The results of CCK-8 and scratch experiment suggested that the proliferation and migration in the pcDNA-LncRNA XR＿378418 group significantly increased. Furthermore, the high-throughput sequencing analysis showed that a total of 248 genes were screened by gene differential analysis, of which 127 were up-regulated, and 117 were down-regulated. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analyses revealed that LncRNA XR＿378418 could regulate cell adhesion, autophagy and Ca2+signaling, etc. Conclusion LncRNA XR＿378418 promotes the proliferation and migration of JS-1 cells and affects the expression of genes related to cell adhesion and calcium signaling in JS-1 cells.