〔Abstract〕 Objective To explore the effect of PAT family proteins homolog 1(PATL1) on the proliferation and migration of gastric cancer cells and its potential mechanism. Methods The expression levels ofPATL1in pancarcinoma, gastric cancer and normal tissues were analyzed by TCGA database. The expression level ofPATL1in 40 human gastric cancer tissues and paired adjacent tissues was evaluated by quantitative real-time PCR(RT-qPCR). The Kaplan-Meier Plotter database was used to analyze the prognosis of PATL1 in gastric cancer patients. The gastric cancer cell line AGS was transfected with PATL1 interference vector, and the interference effect was evaluated by RT-qPCR. The effects ofPATL1on the proliferation and migration of AGS were detected by cell counting kit-8(CCK-8), Transwell test and scratch healing test. The effects of interference withPATL1on the expression of cellular-myelocytomatosis viral oncogene(c-Myc) and autophagy related 7(ATG7) proteins in gastric cancer cells were detected by Western blot assay. Results RT-qPCR showed that the expression ofPATL1in human gastric cancer tissue was higher than that in normal gastric tissue(P<0.001), andPATL1was correlated with the prognosis of patients with enteric gastric cancer(P<0.000 1). AfterPATL1was knocked down, the number of proliferating and migrating gastric cancer cells decreased(P<0.05). Western blot test results showed that the expression level of ATG7 protein decreased afterPATL1was knocked down(P<0.05). Conclusion PATL1 may inhibit the proliferation and migration of gastric cancer cells through crosstalk with c-Myc and ATG7.