〔Abstract〕 Objective To analyze the effects of Porphyromonas gingivalis(Pg) infection and expression of vitamin D pathway-related proteins on the survival and prognosis of patients with esophageal squamous cell carcinoma(ESCC).Methods Pg infection and the expression of 24 hydroxylase(CYP24A1), 1α hydroxylase(CYP27B1) and vitamin D receptor(VDR) in 173 ESCC tissues were detected by immunohistochemistry. The correlation between each index and the survival time of patients was analyzed.Results The positive rates of Pg, CYP24A1, CYP27B1 and VDR in ESCC were 43. 35%, 37. 57%, 20. 23% and 21. 97%, respectively. The 5-year survival time of ESCC patients in the Pg+CYP24A1+CYP27B-VDR-high-risk group was shortened(P<0. 05).Conclusion Pg infection and vitamin D pathway-associated proteins can be used as reliable indicators to predict the survival and prognosis of ESCC patients.