〔Abstract〕 Objective To investigate the mechanism of micellar curcumol(MC) regulating the immune microenvironment of ovarian cancer by promoting the polarization of M2-type macrophages to M1-type in ovarian cancer ascites. Methods(1) After the mice were divided into groups, a mouse ovarian cancer ascites model was constructed by using the mouse ovarian cancer cell line ID8. Then weight changes were observed, tumor tissue and ascites were collected. The expression of CD86 and CD206 on macrophages of the tumor tissue and ascites was detected by flow cytometry. The expression of protein kinase B(PKB/Akt)/mammalian target of rapamycin(mTOR) was detected by Western blot.(2) A human monocytic leukemia cell line(THP-1) was induced to transform into M2 macrophage(THP-1 M2 macrophage) in vitro, and then treated with 10 μg/ml MC. The apoptosis was detected by flow cytometry. The mRNA levels of macrophage mannose receptor(CD206), transforming growth factor-β(TGF-β), interleukin(IL)-1β and tumor necrosis factor-α(TNF-α) were detected by qRT-PCR. The expression of CD86 and CD206 was detected by flow cytometry, and Akt/mTOR expression and phosphorylation was detected by Western blot.Results(1)In vitrostudy showed that the average body weight of the MC group was lower than that of the control group. Compared with the control group, CD206 expression of macrophages decreased in tumor tissue and ascites in the MC group, while the expression of CD86 increased. The Akt and mTOR phosphorylation level of macrophages in the MC group′s ascites was lower than that in control group.(2)In vivostudy showed that there was no difference in apoptosis rate among the groups detected by flow cytometry. The mRNA expression level of CD206,TGF-β and the protein expression level of CD206 in MC group were significantly lower than those in the control group, while the mRNA expression of IL-1β, TNF-α and the protein expression level of CD86 were significantly higher than those in the control group. Compared with the control group, the phosphorylation level of Akt and mTOR in the MC group decreased.Conclusion MC promotes M1 polarization of macrophages in ascites to regulate the immune microenvironment of ovarian cancer, which may be related to the Akt/mTOR pathway.