〔Abstract〕 Objective To observe the expression of Galectin-3 in peritoneal dialysis(PD) fluid in patients with different dialysis ages, and to conduct correlation analysis with vascular endothelial growth factor(VEGF), fibronectin(FN) and related clinical indicators. Methods A total of 109 PD patients who were regularly followed up in the department of nephrology were divided into four groups according to different peritoneal dialysis ages. The concentrations of Galectin-3, VEGF and FN were determined by enzyme-linked immunosorbent assay. The expression of Galectin-3 in peritoneal dialysate of the 4 groups was compared, the correlation with VEGF, FN and clinical related indexes was analyzed, and the correlation was analyzed by Spearman test. Results The concentration of VEGF in peritoneal dialysis patients in group D significantly increased(P<0.05). Galectin-3 expression levels were positively correlated with VEGF(r=0.358,P=0.022), but not significantly correlated with FN(r=0.121,P=0.452). Galectin-3 was positively correlated with clinical indicators parathyroid hormone(PTH)(r=0.201,P=0.037), C-reactive protein(CRP)(r=0.357,P<0.001), left ventricular posterior wall dimensions(LVPWD)(r=0.213,P=0.026), and negatively correlated with clinical indicators total cholesterol(TC)(r=-0.316,P=0.001). Conclusion The concentration of Galectin-3 in the dialysate of long-term peritoneal dialysis patients is significantly elevated, indicating that the expression of galectin-3 increases with the extension of peritoneal dialysis time, suggesting that the detection of galectin-3 levels may be helpful for the evaluation of early peritoneal fibrosis. The positive correlation with VEGF may suggest its role in promoting peritoneal angiogenesis and fibrosis. Moreover, it is positively correlated with clinical indicators PTH, CRP and LVPWD, suggesting that it has certain clinical guiding significance on microinflammatory state and myocardial remodeling.