Investigation the association between γδT cells with endometrial polyps

Acta Universitatis Medicinalis Anhui 2024 06 v.59 971-976     font:big middle small

Found programs:

Authors:Meng Zihan; Yang Yulu; Zou Huijuan; Zhang Xueke; Yang Wenjuan; Sun Dongying; Xu Xiaofeng

Keywords:γδT cells;endometrial polyps;interleukin-17;interferon-γ;inflammatory reaction;embryo implantation failure;infertility

DOI:10.19405/j.cnki.issn1000-1492.2024.06.009

〔Abstract〕 Objective To investigate the expression and clinical value of γδT cells and related inflammatory factors in endometrial polyps(EP).Methods Subjects were patients with infertility due to EP(EP group,n=57) and infertility due to non-uterine factors(Control group,n=44).EP tissues and normal endometrial tissues were taken,respectively.The general characteristics of the two groups were evaluated by independent samples T-test or Wilcoxon rank sum test.The expressions of CD3 and TCRγδ in proliferative endometrium and endometrial polyps were observed by immunofluorescence,and the expressions of IL-17 and IFN-γ in tissue homogenate were detected by ELISA.Uni-variable linear regression was used to analyze the correlation between the number of γδT cells and the expression level of IL-17 and IFN-γ.Results Both the number of γδT cells and he levels of IL-17 and IFN-γsignificantly increased in endometrial polyp tissues,and the relationship between is linear(all P<0.05).Conclusion γδT cells are associated with EP among infertile women,which may lead to embryo implantation failure by affecting the expression of inflammatory factors and regulating the imbalance of cell subsets.