〔Abstract〕 Objective To investigate the effect of growth hormone(GH) pretreatment on the improvement of euploid and pregnancy outcome. Methods A prospective analysis was conducted on 134 patients undergoing preimplantation genetic testing for aneuploidy(PGT-A), among whom 30 patients were self-controlled and 104 patients were inter-group controlled. According to whether GH was added, the patients were divided into GH pretreatment group and GH non-pretreatment group. GH pretreatment included subcutaneous injection of GH 2U/day for 4-6 weeks before the start of gonadotropin(Gn), and the dose was doubled on the day of Gn until the trigger day. GH non-pretreatment meant no GH treatment, GH pretreatment was given when the previous PGT-A cycle failed within one year when the PGT-A was performed again, forming the self-control group.The basic situation, blastocyst situation and pregnancy outcome were compared between the groups by inter-group and self-control. Results No matter in the group control or self-controlled group, the endometrial thickness on the day of HCG, ovarian sensitivity index(OSI), number of oocytes obtained, MII oocytes, 2PN number, 2PN fertilization rate, available oocyte rate, number of biopsy blastocysts, number of euploid blastocysts, euploid blastocyst rate, and at least one euploid rate significantly increased after GH pretreatment, with statistically significant differences(P<0.05). The total amount of Gn, Gn days, number of mosaic blastocysts, and mosaic blastocyst rate were not significantly changed after GH pretreatment, with no statistically significant differences. The implantation rate and clinical pregnancy rate increased after GH pretreatment, but with no statistically significant differences. Conclusion GH pretreatment can significantly improve the number and rate of euploid embryos in patients undergoing PGT-A, and has a tendency to improve pregnancy outcome.