〔Abstract〕 Objective To evaluate the effect of maternal hypothyroidism on lipid metabolism in the early life offemale offspring by constructing a murine model during pregnancy. Methods Female mice C57BL/6J were randomly divided into normal control group(NC0),hypothyroidism complicated with pregnancy group(PH0) and pregnancy complicated with hypothyroidism group(GH0).Firstly, PH0 group was given 0.15% propylthiouracil diet for 28 days, while NC0 and GH0 groups were given normal diet.After the successful modeling of PH0 was confirmed, female mice in GH0 and PH0 groups were given 0.15% propylthiouracil diet during pregnancy, and NC0 group was still given normal diet.After delivery, the levels of free triiodothyronine(FT3),free thyroxine(FT4),thyroid stimulating hormone(TSH) and total cholesterol(TC),triglyceride(TG) in each group were detected at 3 weeks of age for female offspring.The pathological changes of thyroid and liver were observed by HE staining.Western blot detected the levels of proteins associated with lipid metabolism. Results Compared with the NC0 group, the levels of serum FT3 and FT4 decreased, the levels of serum TSH,serum TC,hepatic TC and TG increased in the female offspring of the PH0 and GH0 groups, and lipid deposition was observed in their livers.The expression levels of HMGCR,FAS and CD36 related to lipid metabolism in livers increased, while the expressions of CPT1A and LDL-R were down-regulated. Conclusion Hypothyroidism during pregnancy may lead to the disturbance of lipid metabolism in the early life of female offspring mice.