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Authors：Objective To investigate the effect of miR-135a-5p on the expression levels of insulin-like factor 3(INSL3) and testosterone in the testicular tissues of flutamide-induced cryptorchidism mouse. Methods A model of flutamide-induced cryptorchidism in mouse was constructed, and the experiment was divided into normal control group, flutamide group, flutamide + miR-135a-5p knockdown group and flutamide + miR-135a-5p overexpression group.RT-qPCR was used to detect the expression levels of miR-135a-5p and INSL3 mRNA.Western blot was employed to assess the protein expression level of INSL3.ELISA was performed to measure the expression level of testosterone. Results The expression levels of miR-135a-5p, INSL3 mRNA and protein and testosterone were significantly down-regulated in the testis of cryptorchid mice by flutamide(P<0.05).Knockdown of miR-135a-5p could downregulate the expression of INSL3 mRNA,INSL3 protein and testosterone(P<0.01),while overexpression of miR-135a-5p had the opposite result. Conclusion miR-135a-5p decreased in flutamide-induced cryptorchidism mouse testicular tissues, and overexpression of miR-135a-5p could restore the expression levels of INSL3 and testosterone.

Keywords：Yang Yuangui, An Nini, Chen Hui, He Guoqing, Zhan Xiong, Wu Moudong, Wang Dan, Wang Wei, Peng Jinpu

DOI：10.19405/j.cnki.issn1000-1492.2023.10.009