〔Abstract〕 Objective To investigate the effects of luteolin on invasion and migration of endometriosis stromal cells and whether its mechanism is related to the regulation of 15-hydroxyprostaglandin dehydrogenase(HPGD) expression. Methods The endometrial stromal cells HEM15A were divided into control group(cells were cultured in normal medium),luteolin group(cells were treated with different concentrations of luteolin),si-HPGD group(cells were transfected with si-HPGD),si-NC group(cells were transfected with si-NC),luteolin + si-HPGD Group(cells were transfected with si-HPGD and treated with 20 μmol/L luteolin),Luteolin + si-NC Group(cells were transfected with si-NC and treated with 20 μmol/L luteolin).Real-time quantitative PCR was used to detect mRNA level of HPGD.Cell proliferation was detected by CCK-8 assay, Transwell and scratch assays were used to detect cell invasion and migration.The protein expression of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase(MMP-2),MMP-9 and HPGD were detected by Western blot, and the level of prostaglandin E2(PGE2) was detected by ELISA. Results Compared with 0 μmol/L luteolin, luteolin at 20,50 and 100 μmol/L significantly inhibited the proliferation activity of hEM15A cells, and reduced PCNA expression(allP<0.05).Compared with the control group, 20 μmol/L of luteolin significantly inhibited cell invasion and migration(P<0.05),decreased the expressions of MMP-2 and MMP-9(P<0.05),and up-regulated the mRNA and protein levels of HPGD(P<0.05),while inhibited cellular PGE2 level(P<0.05).Compared with the luteolin group, the luteolin + si-HPGD group increased cell invasion and migration(P<0.05),increased the expressions of MMP-2 and MMP-9(P<0.05). Conclusion Luteolin regulates HPGD/PGE2 signaling pathway to inhibit the invasion and migration of endometrial stromal cells.