〔Abstract〕 Objective To explore the effect of sericin on podocyte epithelial-mesenchymal transition induced by high glucose. Methods Conditional immortalized mouse podocytes were used as the research object to construct podocyte injury model by high glucose stimulation. The podocytes were divided into normal( NG) group,hypertonic control( MG) group,high glucose injury( HG) group,low,medium and high dose sericin( LS,MS,HS)group. CCK-8 method was used to determine the viability of podocytes in each group; the morphological structure of podocytes in each group was observed by ordinary optical inverted microscope. The migration ability was detected by Transwell and cell scratch test. Western blot was used to detect the expression levels of EMT-related mesenchymal phenotype factors Snai1,α-SMA,FSP-1,MMP-9 and epithelial phenotype factors Nephrin,E-cadherin,and WT-1 in podocytes of each group. Results Compared with NG group,HG group showed lower podocyte activity( P<0. 01),worse podocyte status and enhanced podocyte migration ability,up-regulated Snai1,α-SMA,FSP-1and MMP-9 protein expressions( P<0. 01),and down-regulated Nephrin,E-cadherin and WT-1 protein expressions( P<0. 01). Compared with HG group,the viability of podocytes in sericin group increased( P<0. 05),the state of podocytes was improved,the migration ability of podocytes was weakened,the expression of Snai1,α-SMA,FSP-1 and MMP-9 was down-regulated( P<0. 05),and the expression of Nephrin,E-cadherin and WT-1was up-regulated( P<0. 05). Conclusion Sericin can alleviate podocyte epithelial-mesenchymal transition induced by high glucose.