〔Abstract〕 Objective To screen differentially expressed miRNAs and explore its effect and mechanism on cell migration in lung adenocarcinoma(LUAD). Methods Differentially expressed miRNAs in LUAD tissues and normal lung tissues were screened by miRNA microarrays, and then bioinformatics analysis was used to predict their potential biological functions and signaling pathways. The cancer genome atlas(TCGA) analysis and quantitative real-time PCR(qRT-PCR) verified the expression level of hsa-let-7 e-5 p in LUAD tissues and cell lines. The effect of hsa-let-7 e-5 p cell migration in LUAD was examined by would healing experiment. After screening the underlying target genes by bioinformatics analysis, the targeting relationship between hsa-let-7 e-5 p and DTX2,NME6, C8 orf58, GATM and DHX57 were verified by qRT-PCR. Results The miRNA microarray results showed that 347 miRNAs were down-regulated while 229 miRNAs were up-regulated in lung adenocarcinoma tissues. Compared with normal lung tissue and cells, the expression level of hsa-let-7 e-5 p was significantly down-regulated. Besides, overexpression of hsa-let-7 e-5 p inhibitedLUAD cell migration. Conclusion Non-coding RNA hsa-let-7 e-5 p is down-regulated in LUAD and inhibits the migration of lung adenocarcinoma cells. DTX2, NME6, C8 orf58, GATM and DHX57 are the potential target genes of hsa-let-7 e-5 p.