〔Abstract〕 Abstract: Objective To study the preparation method of silane and bone morphogenetic protein-2(BMP-2) on the nano-porous titanium surface and evaluate the osteogenic ability of the prepared surface. Methods Silane and BMP-2 modified nano-porous titanium surfaces were constructed by alkali-heat treatment, silanization modification and BMP-2 grafting. Scanning electron microscopy, X-ray photoelectron spectroscopy, infrared spectroscopy and water contact angle tests were used to characterize the changes of surface morphology, elements, functional groups and hydrophilicity during the preparation process. Immunofluorescence was used to observe the BMP-2 grafted on the surface. Cell activity test and cell fluorescence staining were used to analyze the ability to promote osteoblast adhesion and proliferation, and alkaline phosphatase activity was used to evaluate the ability to promote cell osteogenesis. Results Material characterization results confirmed that silane and BMP-2 were successfully immobilized on the nano-porous titanium surface.Cell evaluation confirmed that the prepared surface not only significantly enhanced the adhesion and proliferation of osteoblasts, but also greatly enhanced the osteogenic ability of osteoblasts. Conclusion The constructed surface has better compatibility with osteoblasts and is expected to improve the osseointegration ability of titanium-based implants.