〔Abstract〕 Objective To construct the sodium alginate-chitosan polyelectrolyte multilayer membrane structure on the surface of alkaline heat treated titanium by LBL technology, and to combine the peptide that specifically bind to osteoblasts with this structure to explore their slow-release effects. Methods After the smooth titanium surface was heat treated with NaOH, sodium alginate and chitosan were alternately adsorbed and deposited on the surface by layer by layer self-assembly method to form a polyelectrolyte multilayer film structure, and the multilayer film was used to load the peptide that specifically bind to osteoblasts, construct slow-release system, and evaluate the slow-release effect. Results The sample surface was tested by contact angle measuring instrument, field emission scanning electron microscope, fluorescence microscope, X-ray photoelectron spectrometer, Fourier transform infrared spectroscopy and ultraviolet spectrophotometer to confirm that the sodium alginate-chitosan multilayer film structure was successfully constructed on the surface of titanium sheets, and the peptide that specifically bind to osteoblasts were successfully loaded. Conclusion The surface of titanium implants is self-assembled layer by layer to form a multilayer membrane structure and successfully loaded with the peptide that specifically bind to osteoblasts, and the sustained release system of the peptide is constructed, which has a remarkable sustained release effect and is expected to promote osseointegration on the surface of titanium implants.