〔Abstract〕 Objective To investigate the expression and clinical significance of classic vascular endothelial growth factor A(VEGFA) and vasotropic mimicry factor matrix metalloproteinase 14(MMP-14) in lung adenocarcinoma(LUAD). Methods The expression levels of MMP-14 and VEGFA genes and proteins in LUAD and their correlation with survival and prognosis were analyzed using TCGA and UALCAN databases. Serum of 69 patients with LUAD and 20 healthy subjects(control group) was collected, and the contents of MMP-14 and VEGFA were detected by ELISA and chemiluminescence, respectively, to analyze the correlation between the two and the clinicopathological features of tumors and their value in prediction and diagnosis of LUAD. Results The levels of MMP-14 and VEGFA in LUAD tissues and serum were higher than those in control group. There were significant differences in serum VEGFA and MMP-14 expression levels among early stage group, advanced stage group and control group(P<0.001). MMP-14 was higher in T3/T4stage than that in T1/T2stage(P=0.045), higher in N2/N3stage than that in N0/N1stage(P=0.035), and higher in the pleural metastasis group than that in the non-pleural metastasis group(P=0.034). VEGFA level was higher in M1than M0(P=0.025). Elevated VEGFA level was a risk factor for LUAD(P=0.002). The area under the curve(AUC) of MMP-14, VEGFA and CEA alone was 0.793,0.849 and 0.851, respectively, and the AUC of the combined test was 0.952. The overall survival(OS) and disease specific survival(DSS) of MMP-14 and VEGFA low expression group were longer than those of MMP-14 and VEGFA high expression group(P<0.05). Conclusion High expression of MMP-14 and VEGFA in LUAD is associated with the growth, invasion and metastasis of LUAD, respectively, and has implications for survival prognosis determination.