〔Abstract〕 Objective To investigate the efficacy of standardized allergen subcutaneous immunotherapy(SCIT) in children with asthma sensitized to single dust mite allergens versus multiple allergens and to assess the safety of SCIT. Methods 62 children with confirmed allergic asthma who received standardized allergen SCIT were retrospectively analyzed and divided into the monosensitized group(dust mite results≥+++) and the polysensitized group(dust mite results ≥+++ combined with other positive allergens) according to the results of skin prick test, we observed the changes of pulmonary function, medication score and visual analog scale(VAS) scores, children asthma control test(C-ACT)scores, asthma control questionnaire(ACQ) scores before and after treatment in both groupsand compared the efficacy of the two groups. The incidence of local and systemic adverse effects was recorded during treatment in all children to assess the safety of SCIT. Results Standardized allergen SCIT treatment improved lung function parameters, medication scores and VAS scores, C-ACT scores, ACQ scores in both the monosensitized and polysensitized groups, with statistically significant differences before and after treatment(P<0.05). In comparison between the two groups, lung function parameters [forced expiratory flow at 50% vital capacity(FEF50%), maximum midexpiratory flow(MMEF)], medication scores, C-ACT scores and ACQ scores improved significantly in the monosensitized group compared with the polysensitized group after treatment(P<0.001). 62 patients received a total of 2 606 injections during the treatment of SCIT, 6 children had a total of 10 local adverse reactions and 3 children had 3 mild to moderate systemic adverse reactions, with an incidence of 0.38% for local adverse reactions and 0.12% for systemic adverse reactions. Conclusion The children with asthma in both the monosensitized group and polysensitized group achieved significant and safe clinical outcomes under standardized allergen SCIT. The children in the monosensitized group had more obvious clinical effects than the polysensitized group under standardized allergen SCIT.