〔Abstract〕 Objective To clarify the targeted regulatory relationship between long non-coding RNA(lncRNAs) lnc-CCDC117-1 and oncogenic transcription factor c-Myc, and to explore the effect of lnc-CCDC117-1 knockdown and overexpression on the development of tongue squamous cell carcinoma cells. Methods Lentiviral vectors carrying flag-c-Myc, plko.1-shc-Myc and their controls were transfected into HN6,SCC9 and CAL27 cells respectively, and real-time quantitative PCR(qRT-PCR) was used to detect the expression of lnc-CCDC117-1. In addition, the intracellular localization of lnc-CCDC117-1 was detected by fluorescence in situ hybridization. On this basis, the lnc-CCDC117-1 expression vector and knockdown vector were constructed and transfected with HN6, SCC9, CAL27 cells, CCK-8 method, cloning and formation to verify the proliferation of tongue squamous cell. Results LncRNAs positively regulated by c-Myc were initially screened by gene chip technology. After overexpression or c-Myc knockdown in tongue squamous cell HN6, these lncRNAs were verified to be consistent with the results of the chip, and the expression difference of lnc-CCDC117-1 was found to be the most significant. qRT-PCR test showed that c-Myc had a positive regulatory effect on lnc-CCDC117-1. When c-Myc was overexpressed, the expression of lnc-CCDC117-1 could be significantly up-regulated. lnc-CCDC117-1 expression was significantly down-regulated by c-Myc knockdown. Dual luciferase reporter genes showed that c-Myc could target the regulation of lnc-CCDC117-1, and c-Myc was involved in regulating and enhancing the transcriptional activity of lnc-CCDC117-1. lnc-CCDC117-1 mainly existed in the nucleus of the cell. qRT-PCR results showed that the expression of lnc-CCDC117-1 and c-Myc significantly decreased by sh-lnc-CCDC117-1. The expression of lnc-CCDC117-1 was significantly up-regulated by +lnc-CCDC117-1. The results of growth curve assay, CCK-8 assay, cell scratch assay and cloning formation showed that overexpression of lnc-CCDC117-1 significantly promoted the proliferation and migration of tongue squamous cell cells, while knockdown of lnc-CCDC117-1 could inhibit the growth and proliferation of tongue squamous cell carcinoma cells. Conclusion lnc-CCDC117-1 is positively regulated by c-Myc, and overexpression of lnc-CCDC117-1 can promote cell proliferation, on the contrary, inhibit cells growth.